中药鬼箭羽降糖有效部位的药效学和化学研究

Hypoglycemic Effects of Extracts and Constituents from Euonymus alatus

目的 :在对中药鬼箭羽药效学研究基础上 ,提取分离其降糖作用的物质基础。方法 :用健康小鼠和四氧嘧啶所致的化学性糖尿病小鼠模型 ,以血糖为指标研究鬼箭羽的降糖作用有效部位和物质基础 ,采用硅胶和凝胶柱层析等分离纯化技术 ,根据理化性质及光谱数据鉴定结构。结果 :鬼箭羽对健康小鼠血糖水平无明显影响 ,但能显著降低四氧嘧啶所致糖尿病小鼠血糖水平 ,其中水提取部位降糖作用最好。乙酸乙酯部位降糖作用仅次于水提取部位 ,乙酸乙酯部位经聚酰胺柱色谱用 5 0 %乙醇洗脱所得组分降糖效果明显。对 5 0 %乙醇洗脱组分进行分离得到 4个酚酸类化合物 ,分别为对羟基苯甲酸 (p hydroxybenzoicacid ,EA 1) ,3,4 二羟基苯甲酸 (protocatechuicacid ,EA 2 ) ,3 甲氧基 4 羟基苯甲酸 (4 hydroxy 3 methoxybenzoicacid ,EA 3) ,3 5 二甲氧基 4 羟基苯甲酸 (3,5 dimethoxy 4 hydroxybenzoicacid ,EA 4 )。结论 :鬼箭羽对糖尿病小鼠有明显的降糖作用 ,其活性物质主要来自于乙酸乙酯部位 5 0 %乙醇洗脱所得的化学组分。从该部位分离的四个成分均为首次从该植物中分得。

To observe the hypoglycemic effects o f Euonymus alatus (Thunb.) Sieb and to determine its hypoglycemic effective extract. Normal mice and alloxan induced diabetic mice were used to observe the hypoglycemic effects.Ethyl acetate ex tract o f Euonymus alatus shows hypoglycemic effects. Four compounds were isolat ed from a ctive groups and they were identified as p hydroxybenzoic acid,(EA 1),pr otocatech uic acid (EA 2),4 hydroxy 3 methoxybenzoic acid,(EA 3) and 3,5 dimetho xy 4 hydr oxybenzoic acid,(EA 4). The fasting serum gl ucose le vel in diabetic mice was reduced significantly, but similar effect on fasting se rum glucose level was not produced in normal mice.EA 1～EA 4 were firstly isolated from Euonymus alatus .