AXL基因在骨髓增生异常综合征外周血单个核细胞中的表达

AXL gene,a receptor tyrosine kinase expressed in the peripheral mon

目的 探讨AXL基因在正常人、骨髓增生异常综合征 (MDS)及其他血液病患者外周血单个核细胞中的表达情况。方法 采用RT PCR法。结果 ①AXL基因在 10例正常人外周血单个核细胞中不表达 ,正常骨髓单个核细胞中有表达。② 5 0例MDS患者的外周血单个核细胞中有 4 1例 (82 % )AXL基因阳性。FAB亚型与AXL表达关系 :3 5例难治性贫血 (RA)者中 2 7例阳性 ,3例环形铁粒幼细胞性难治性贫血 (RAS)阳性 ;9例难治性贫血伴过多母细胞 (RAEB)者中 8例阳性 ,3例难治性贫血伴过多母细胞伴有转化 (RAEB T)患者均阳性。 6例实验时临床不能确诊为MDS者AXL基因阳性 ,经随访骨髓转为MDS ;3例骨髓疑为MDS或诊为MDS者AXL基因阴性 ,经随访确诊为其他疾病。③ 12例再生障碍性贫血 (AA)、1例缺铁性贫血 (IA)、1例溶血性贫血、2例夜间阵发性血红蛋白尿 (PNH)、3例特发性血小板减少性紫癜 (ITP)患者的AXL均阴性。

Objective To study the expression of AXL gene in the peripheral mononuclear blood cells of MDS and several kinds of hemotologic disorders.Methods The mRNA of AXL from mononuclear cells of the patients peripheral blood was analyzed by RT PCR method.Results ①10 cases of normal control group showed negative expression;②41 cases from 50 cases of MDS(82%) had positive AXL expression in the peripheral mononuclear blood cells.The relationship of FAB subtype and AXL expression was:3 cases of RAS,8 cases in 9 cases of RAEB,3 cases of RAEB T all showed positive expression.6 patients who showed positive expression could not be diagnosed according to the FAB standards at the beginnig,but they turned to be typical MDS as follow up.3 patients being thought to be MDS who had negative AXL gene expression were other hematologic disorder after follow up;③There were no AXL expression in peripheral blood of patients with AA,IA,HA,PNH and ITP.Conclusions AXL gene shows high expression in the peripheral blood cells of patients with MDS but no expressions in AA,PNH and etc,it may be one of the specific and sensitive indication in MDS.