摘要
以间接标记法首先合成中间体对砹苯甲酸,再通过活化羧基反应联接到3H_(11)McAb上,制得^(211)At-3H_(11)McAb偶联物,放射性比度为18.5—61.1kBq/μg。ELISA法鉴定标记后的^(211)At-3H_(11)McAb的免疫活性与未标记3H_(11)McAb一致。实验表明:^(211)At-3H_(11)McAb对靶细胞杀伤效应高于^(211)At-IgG及Na^(211)At;当靶细胞先与一定量3H_(11)McAb或3G_9McAb(4μg)作用1h,可抑制^(211)At-3H_(11)McAb对靶细胞的杀伤效应,放射性比度不影响杀伤效应。
The synthesis of 211 At labelled 3H11 monoclonal antibody was performed by an activation reaction of carboxy via paraastato - benzoic acid intermediate. Its specific radioactivity was 18. 5-61. 1kBq/μg . Its immunoactivity was measured by ELISA method. In vitro binding and cytotoxic experiments showed when the 211At - 3H11McAb concentration is raised, the cytotoxic effect increased and have obvious selectivity. The cytotoxic effect of 211At - 3H11 McAb to target cell M85 was higher than that of 211At - IgG and Na211At . If the M85 cells were cultured with 3H11 McAb or 3G9McAb for 1 h at first, the cytotoxic effect of 211At -3H11McAb was inhibited. The specific radioactivity of 211At - 3H11McAb and cytotoxic effect are also discussed.
出处
《核技术》
CAS
CSCD
北大核心
1992年第11期678-682,共5页
Nuclear Techniques
基金
国家自然科学基金
关键词
放射疗法
免疫疗法
肿瘤
砹同位素
211At - 3H11McAb Cytotoxic effect Immunoac tivity
