碱性成纤维细胞生长因子对海马伞切断大鼠海马神经细胞增殖的影响

Effect of basic fibroblast growth factor on neurogenesis in the dentate gyrus of rat with fimbria-fornix transection

目的 探讨碱性成纤维细胞生成因子 (bFGF)对海马伞切断造成的阿尔茨海默氏病 (AD)模型大鼠海马齿状回神经细胞增殖的影响。方法 AD处理组及AD对照组大鼠单侧海马伞切断制造阿尔茨海默病模型 ;正常对照组注射生理盐水。AD处理组造模后每天侧脑室注射bFGF共 7天 ;AD对照组及正常对照组同时间注射生理盐水。BrdU标记增殖细胞。TUNEL方法标记DNA片段 ,原位检测凋亡细胞。计数海马齿状回各区域BrdU阳性细胞与凋亡细胞数。结果 AD处理组大鼠与AD对照组大鼠相比 ,海马齿状回BrdU阳性细胞增加明显 (P <0 .0 1 ) ,而凋亡细胞差异不显著 (P >0 .0 5)。AD对照组大鼠与正常对照组大鼠相比 ,海马齿状回BrdU阳性细胞数及凋亡细胞数差异均不显著 (P >0 .0 5)。结论 bFGF可促进AD模型大鼠海马神经细胞的增殖。

Objective To study the effect of bFGF on neurogenesis in brain of AD model rat with unilateral fimbria fornix transection. Methods We develop the AD model through unilateral fimbria fornix transection . AD model rats received bFGF or saline by infusion into the cerebroventricular space every day after being lesioned.After 7 days bFGF infusion , 5 BrdU was used to label the proliferation cells after bFGF infusion. TUNEL was used to label the apoptotic cell .Then we count the positive cells labeled for 5 BrdU or TUNEL in the dentate gyrus.Result Comparison to AD control group, the number of 5 BrdU positive cells in the dentate gyrus of AD treatment group was increased significantly .But the number of TUNEL positive cells was no difference from that of the AD control group'. The number of 5 BrdU positive cells and the number of TUNEL positive cells in the dentate gyrus of AD control group were no difference from that of the normal control group'. Conclusion It is showed that bFGF can acceleration the neurogenesis in the dentate gyrus of AD model rat with unilateral fimbria fornix transection.