黄芪对大鼠肾小球系膜细胞增生水平的影响

Effect of Huangqi Injection on the Level of Proliferation of Gromerular Mesangial Cell In Rats

目的 :探讨黄芪注射液 ( AM)对大鼠肾小球系膜细胞 ( GMCs)增生作用的影响。方法 :实验分三组 ,空白对照组 ,脂多糖组 ( LPS) ,综合组 ( LPS+ AM)。采用 MTT掺入方法 ,于 2 4、48小时 ,观察三组 GMCs增生水平。结果 :综合组于 48小时 GMCs增生的抑制最为显著 ,且 GMCs增生水平明显低于脂多糖组 ( P<0 .0 1 )和空白对照组 ( P<0 .0 5 )。结论 :AM能抑制大鼠 GMCs增生。

Objective: For observe the mechanism that AM inhibit proliferation of rat glomerulus mesangial cells(GMCs).Methods:Rat GMCs line was cultured in vitro, Lipoplysaccharide(LPS), LPS and AM were added into the ctfiture medium, establish three experiment groups: GMCs+LPS+AM,GMCs+LPS and GMCs group, respectively. GMCs proliferative rate was detected by MTT.Results: At AM group, the GMCs proliferative rate were lower than that either in LPS group or in GMCs group( P <0.01). Conclusion:It suggests that AM can obviously inhibit proliferation of GMCs.