γ射线照射对浓缩血小板保存中细胞因子含量的影响

Effect of prestorage Gamma radiation on the cytokine levels in stored platelet concentrates

目的 :研究γ射线照射对浓缩血小板保存过程中细胞因子含量的影响。方法 :采用富含血小板血浆 (plateletrichplasmaPRP)法制备浓缩血小板 ,然后以 2 5GY的γ射线照射 ,于标准条件下保存 5d ,间隔取样用ELISA方法检测IL - 1 β、IL - 6、IL - 8及TNFα含量。结果 :对照组浓缩血小板在保存 3d后 ,所有细胞因子含量与保存前比较显著升高 (P <0 .0 5) ,经γ射线照射后保存 3d ,除IL - 8外 ,其它细胞因子含量均与保存前无明显差异 (P >0 .0 5)。但在保存 5d后 ,所有细胞因子均与保存前比较有明显升高 (P <0 .0 5)。结论 :γ射线照射能抑制浓缩血小板保存过程中细胞因子含量的升高 。

Objective:To study the effect of prestorage Gamma radiation on the cytokine levels in platelet concentrates storage.Methods:Platelet concentrates prepared by the apheresis platelet (AP) methods were irradiated by Gamma ray at 25 Gy, then stored at standard condition for 5 days. Cytokine levels were measured by ELISA at various storage time. Results :The contents of all cytokines in platelet concentrates of control group after 3 days storage were significantly different from those prestorage ( P <0.05). The content of all cytokines except IL-8 in platelet concentrates of irradiated group showed no significant changes after 3 days storage ( P >0.05). But after 5 days storage, the content of all cytokine were much higher than those prestorage( P <0.05).Conclusion:Gamma radiation is effective in preventing the generation of cytokines in the storage of platelet concentrates. Thus Gamma radiation at 25 Gy could prevent non-hemolytic febrile reaction after platelet concentrates transfusion.