地黄寡糖灌胃对糖尿病大鼠的降糖作用及对肠道菌群的影响

Hypoglycemic effects and regulation on intestinal flora of Rehmannia glutinosa oligosaccharides in alloxan- induced diabetic rats by oral administration

目的 :观察地黄寡糖灌胃给药对四氧嘧啶 (ALX)糖尿病大鼠的降糖作用和对肠道菌群的影响。方法 :以AL× (1 50mg·kg- 1 ,ip)诱发糖尿病大鼠模型 ,灌胃给予地黄寡糖 2 0 0mg·kg- 1 ·d- 1 ,检测给药前、给药 7d及给药 1 4d后大鼠血糖、血浆胰岛素、肝糖原的变化及给药 1 4d肠道菌群的变化。结果 :给药组大鼠血糖降低、血清胰岛素浓度及肝糖原含量增加 ,肠道菌群中双歧杆菌类杆菌、乳杆菌等优势菌群的数量明显增加 ,与糖尿病模型组比较差异有显著性 (P <0 .0 5)。结论 :地黄寡糖具有降低ALX糖尿病大鼠血糖及调节肠道菌群功能的作用 ,调节机体微生态平衡可能是地黄寡糖降血糖机制之一。

Objective:To investigate the regulatory effects of Rehmannia glutinosa oligosaccharides (ROS) through oral administration on glucose metabolism and relation ship to the intestinal flora in alloxan-induced diabetic rats.Methods:Alloxan-induced diabetic model rats were administrated by i.g. with ROS at a dose of 200 mg·kg -1 . The plasma glucose level , insulin level, hepatic glycogen were determined at d 0, d 7 and d 14 after ROS administration, and intestinal flora was measured at d 14 after ROS administration.Results:The blood glucose level was reduced, plasma insulin level and hepatic glycogen were increased in ROS treated diabetic rats and the number of intestinal flora was significantly higher than that in the control diabetic rats.Conclusion:The results indicated that ROS exerted a hypoglycemic effect and a modulation function on intestinal flora in alloxan-induced diabetic rats.