牙龈卟啉菌、中间普氏菌的超微结构及表面相关物质的电泳特性

Electron microscopic observation and SDS-PAGE analysis of porphyromonas gingivalis ,prevotella intermedia

目的 了解牙龈卟啉菌、中间普氏菌的细胞壁结构及表面蛋白的特性。方法 通过透射电镜观察这两种细菌的超微结构 ,分析两种细菌脂多糖和外膜蛋白的电泳特性。结果 两种细菌的外膜蛋白电泳图各有 4条较清晰的主带 ,分子量分别为 92kDa、5 5kDa、3 5kDa、2 3kDa和 75kDa、5 5kDa、40kDa、18kDa。结论 牙龈卟啉菌和中间普氏菌具有典型革兰氏阴性菌细胞形态 。

Objective To observe the ultrastructure of Porphyromonas gingivalis and Prevotella intermedia. Methods Electron microscopic observe the ultrastrucre of the two bacteria. SDS-PAGE analyses the protein patterns of outer membrances and lipoplysaccharides.Results The two bacteria showed the typical gram-negative cell envelope. The out membrances of Porphyromonas gingivalis and Prevotella intermedia have four protein bands , 92kDa?55kDa?35kDa?23kDa and 75kDa?55kDa?40kDa?18kDa respectively.Conclusion They have the typical gram negative structure. The out membrance proteins are the important pathogenic structures.