人参皂苷Rg1、Rh1对树突状细胞刺激T细胞增殖及LPAK抗肿瘤活性的影响

Effect of Ginsenoside Rg1 and Rh1 on the function of dendritic cell stimulating the proliferation of T cell and anti-tumor activity of LPAK

目的 :观察人参皂苷Rg1,Rh1对树突状细胞 (DC)刺激T细胞增殖及其对IL 2与PHA激活的杀伤细胞 (DC LPAK)对人乳头瘤细胞及L92 9细胞杀伤作用的影响。方法 :分离培养鉴定DC ,用MTT法检测Rg1及Rh1对DC刺激T淋巴细胞增殖作用的影响。用中性红摄入比色法观察DC LPAK的杀伤活性。结果 :T :DC为 10 :1及 2 5 :1时 ,仅Rg110 0 μg ml能促进T细胞增殖 (P <0 0 5 ) ,而Rh110 0 μg ml则抑制T细胞增殖 (P <0 0 1) ,同剂量的Rg1及Rh1相比有显著性差异 (P <0 0 5～0 0 1) ;T :DC为 10 :1时 ,Rh110 μg ml抑制T细胞增殖 ,而在T :DC为 2 5 :1时则表现为促增殖作用 (P <0 0 5 )。T :DC为 5 0 :1时 ,各加药组对T细胞增殖均无作用。Rg1及Rh1均能促进DC LPAK对乳头瘤细胞的杀伤能力 ;在对L92 9杀伤实验中 ,效靶比为 5 :1时 ,Rg1各剂量均能显著促进DC LPAK的杀伤活性 (P <0 0 1～ 0 0 5 ) ,而Rh1仅中剂量能提高DC LPAK的杀伤活力 (P <0 0 5 )。结论 :Rg1可增强DC刺激T细胞增殖及DC LPAK杀伤肿瘤的细胞能力。Rh1的作用则与其剂量范围及细胞比例密切相关。

Objective:To study the effect of Ginsenoside Rg1 and Rh1 on the function of dendritic cell (DC) of stimulating the proliferation of T cell and anti tumor activity of LPAK which was activated by PHA and IL 2 Methods:After the separation, incubation, and the identification of DC, MTT is used to detect the effect of Rg1 and Rh1 on the function of DC in stimulating the proliferation of T cells Anti tumor activity of DC LPAK was determined by neutral red phagocytosis assay Results:Rg1 100 μg/ml can promote the proliferation of T cell (P<0 05) at the T:DC rate 10:1 and 25:1 while the same dose of Rh1 shown opposite effect on T cell proliferation(P<0 01), compared with that of Rg1, the inhibition effect is obvious (P<0 05～0 01); Rh1 10 μg/ml showed inhibition effect on T cell proliferation at T:DC rate of 10:1 while promotion effect at 25:1 Both Rg1 and Rh1 have no effect on T cell proliferation of stimulated by DC Rg1 and Rh1 can enhance the anti tumour ability of DC LPAK on human papillate tumor cell line, each dose of Rg1 can obviously accelerate the cytotoxity on L929 at the E:T ratio of 5:1(P<0 05～0 01), while only Rh1 10 μg/ml can enhance the cytotoxity ability of DC LPAK(P<0 05) Conclusion:Rg1 can promote the ability of DC on stimulating the proliferation of T cell and cytotoxity of DC LPA K, while the effect of Rh1 is in the relation to its dose range and the proportion of target cells