CRF患者红细胞促外周血淋巴细胞增殖反应的实验研究

Study of proliferative reaction of erythrocyte promoting peripheral blood lymphocyte on chronic renal failure patients

目的 :探讨慢性肾功能衰竭 (CRF)患者红细胞 (RBC)促外周血淋巴细胞 (PBL)增殖能力 ,了解CRF患者RBC免疫调控功能状况。方法 :CRF患者 (检测组 )和正常人 (对照组 )的RBC为效应细胞 ,正常人淋巴细胞为靶细胞 ,采用四甲基偶氮唑蓝 (MTT)比色法 ,检测红细胞调控淋巴细胞增殖反应的能力。结果 :健康人RBC促PBL增殖的作用明显 ,其增殖率为5 7 3%± 10 2 % ,而CRF患者RBC促PBL增殖作用较健康人明显低下 ,增殖率为 32 7%± 7 8% (P <0 0 0 1) ,透析后有较明显的改善 ,增殖率为 4 0 6 %± 11 6 % ,但仍低于健康人。结论 :CRF患者红细胞免疫调控功能低下。

Objective:To study the activity of immunological regulative function of red blood cells of the patients with chronic renal failure.Methods:The proliferative reaction of the erythrocyte promoting lymphocyte was detected in CRF patients and healthy persons with MTT colorimetry,in which the normal person's lymphocyte as the target cell and the erythrocyte as the stimulating cell.Results:It was found that the healthy persons of control group had obvious proliferative reaction of the erythrocyte promoting lymphocyte(promoting rate:57.3%±10.2%),while the 36 CRF patients had lower proliferative activity(promoting rate:32.7%±7.8%,P<0.001),and had remarkable improvement after dialysis(promoting rate:40.6%±11.6%) though lower than the healthy persons yet.Conclusion:The CRF patients had lower immunoregulative function of the erythrocyte.