野黄芩甙对发热家兔下丘脑及脑脊液中环磷腺苷含量的影响

Effects of Scutellarin on Contents of cAMP in Hypothalamus and Cerebrospinal Fluid of Rabbits With Fever

目的:从中枢发热介质的角度探讨野黄芩甙的解热机制。方法:本实验用内毒素(ET)复制家兔发热模型,观察野黄芩甙的解热作用及对家兔下丘脑和脑脊液中环磷腺苷(cAMP)含量的影响。结果:静脉注射ET后80 min,野黄芩甙+ET组家兔的体温明显低于ET组(P<0.01),表明野黄芩甙具有明显的解热作用;并且野黄芩甙能抑制ET所致的下丘脑中cAMP含量的升高(P<0.01)和脑脊液中cAMP含量的升高(P<0.01);相关分析显示,下丘脑和脑脊液中cAMP的含量变化与体温变化之间呈明显的正相关(P<0.01)。结论:野黄芩甙可能是通过抑制下丘脑和脑脊液中cAMP含量升高而发挥其解热作用的。

Objective: Our aim was to study the antipyretic mechanism of scutellarin. Methods: Fever models of rabbits were made by injection of endotoxin( ET) in vein. The antipyretic action and effects of scutellarin on contents of cyclic adenosiue monopliosphate(cAMP) in hypothalamus and cerebrospinal fluid(CSF) were observed. Results: Body temperature of rabbits in scutellarin plus ET group were lower than those of ET group at 80 minutes after injection of ET in vein ( P < 0. 01 ) .which showed that scutellarin possessed obvious antipyretic effects on rabbits with fever. Scutellarin might inhibit the increase of contents of cAMP caused by ET in hypothalamus( P < 0.01) and CSF( P < 0.01). Correlation analysis showed that contents of cAMP in hypothalamus and CSF were positively correlated with change of body temperature of rabbits ( P < 0. 01 ). Conclusion :Scuteilarin may have antipyretic effects on rabbits witli fever by inhibiting the increase of contents of cAMP in hypothalamus and CSF.