CD44基因内含子9mRNA在子宫内膜癌中的表达及意义

Expression of Intron 9 of CD44 Gene in Endometrial Carcinoma and Its Clinical Significance

目的:探讨子宫内膜癌组织中CD44基因内含子9mRNA的表达情况及临床意义。方法:采用RT-PCR法检测31例子宫内膜癌组织,10例子宫内膜不典型增生组织,10例正常子宫内膜组织中CD44基因内含子9mRNA的表达情况。结果:正常子宫内膜组织中无CD44内含子9mRNA的表达,子宫内膜癌与子宫内膜不典型增生组织中有CD44内含子9mRNA表达;二者表达率分别为83.87％和70.00％,差异无显著性(P>0.05),相对含量分别为85.26±5.40和79.74±4.23,差异有显著性(P<0.05);在子宫内膜癌中CD44内含子9mRNA的表达情况与其临床病理特征无关(P>0.05)。结论:CD44基因内含子9表达与子宫内膜癌的早期发生有关。

Objective: Our aim was to study the expression of intron 9 of CD44 gene in endometrial carcinoma and its clinical significance. Methods: The expression of intron 9 was detected in 31 cases of endometrial carcinoma ( EC) , 10 of atypical hyperplasia endometrium (AH) , and 10 of normal endometriuni (NE) with reverse transcription polymerase chain reaction (RT-PCR). Results: No expresssion of intron 9 was found in NE. The expression rates of intron 9 in EC and AH were 83. 87% and 70. 00% , respectively, and they were not significantly different ( P >0.05). Their relative contents of intron 9 were 85. 26 ± 5.40 and 79.74 ± 4. 23 and the difference was significant ( P < 0.05). There was no relationship between the expression of intron 9 and the clinical pathological characteristics. Conclusion: The expression of intron 9 in gene CD44 was related to the carcinogenesis of EC.