新生儿期血浆中神经节苷脂测定

Determination of Plasma Ganglioside Concentration in Neonates

目的:观察新生儿血浆中神经节苷脂动态变化,评价其对新生儿生长发育的影响。方法:应用改良间苯二酚盐酸微量法测定血浆中总神经节苷脂结合的唾液酸含量。结果:血浆总神经节苷脂结合的唾液酸含量在出生时最低,为(7.80±0.79)nmoL/ml,14 d达高峰,为(16.25±1.44)nmoL/ml,与出生时相比,显著增高,P<0.01。1岁时为(12.22±1.19)nmol/ml,与日龄14 d相比。显著减低,P<0.01;与出生时相比,明显增高,P<0.01。日龄14 d后随日龄增加,血浆总神经节苷脂结合的唾液酸含量逐渐减低(Y=15.36-0.007X+eps,r=-0.53,P<0.01)。结论:新生儿血浆中神经节苷脂水平至少在新生儿期可以反映中枢神经系统的发育与成熟。

Objective: Our aims were to observe tiie changes of ganglioside in plasma of neonates and to evaluate the clinical significance. Methods: Total ganglioside (lipid-bound sialic acid, LBSA) concentration in plasma were quan-titativley determined by using an improved micro-method of resorcinol-hydrochloride staining. Results: The mean plasma ganglioside concentration ( LBSA) at birth was (7. 8±0. 79) nmol/ml. It increased rapidly early in the neonatal period and reached the maximum level at 14 days ( 16. 25±1. 44)nmol/ml, significantly higher than the value at birth, (P < 0. 01). Then it decreased gradually to (12.22 ± 1. 19)nmol/ml at one years old, which was lower than the value at 14 days, (P < 0. 01) ; and higher than the value at birth (P < 0. 01), Plasma ganglioside concentration decreased from 14 days of age significantly ( Y = 15. 36 - 0. 007X + eps, r = - 0. 53, P < 0. 01). Conclusion: Plasma ganglioside concentration might reflect the development and maturation of the central nervous system to some degree, at least early in the neonatal period.