下颌骨骨折愈合过程中整合素α_5表达的免疫组化研究

Immunohistochemical Research of Integrin α_5 Expression during Mandibular Fracture Healing

目的 探讨下颌骨骨折愈合过程中整合素α5表达水平的改变。方法 用兔作下颌骨骨折的动物模型 ,分别于骨折前 1天及骨折后第 1、3、5、7、14、30、6 0、90天取骨断端组织标本作成石蜡切片并用LsAB法进行免疫组织化学实验 ,以探讨整合素α5在骨组织 ,特别是在成骨细胞和破骨细胞中的表达。结果 整合素α5在骨组织及其周围软组织中广泛表达 ;从骨折后第 7天出现增强 ,第 14天到第 30天表达最强 ,第 6 0～第 90天后接近正常。结论 在下颌骨骨折的愈合过程中 ,整合素α5在骨组织细胞上的表达增强 ,整合素α5对骨折的愈合过程具有重要作用。

Objective\ To investigate the expression change of integrin α\-5 during mandibular fracture healing.Methods\ Using rabbit mandibular fracture model, the fractured bone tissues were obtained and paraffin slices were made on the days of 1,3,5,7,14,30,60 and 90 after surgery, respectively. Non_fractured mandibles were used as normal control. LsAB immunohistochemical method was used to detect the expression of integrin α\-5 in bone tissue, especially on osteoblasts and osteoclasts.Results\ Integrin α\-5 was widely expressed in fractured bone and surrounding soft tissues. The expression of integrin α\-5 increased 7 days after fracture, peaked in 14 to 30 days and returned to nearly normal in 60_90 days.Conclusion\ During mandibular fracture healing, the expression of integrin α\-5 in bone tissue increased clearly. It is estimated that integrin α\-5 playes an important role in fracture healing.\;