枳椇子水提取物细胞毒作用与抑瘤功效的研究

The study of cytotoxic effect and antitumor effect by Hovenia dulcis Thunb

目的 :考察枳木具子水提取物体外细胞毒作用和体内抑瘤作用。方法 :噻唑兰 (MTT)体外实验法和灌胃给药体内抑瘤实验。结果 :2 .5～ 10 0mg/ml枳木具子水提取物对体外培养的人肝癌Bel- 74 0 2细胞的生长呈抑制作用 ,ID50 14 .0mg/ml;体内灌胃给予枳木具子水提取物 2 ,0 .4 ,0 .0 8g生药 /kg·d× 10天剂量下 ,对小鼠肿瘤H2 2的抑制率依次为 2 5 .4 1% ,36 .95 % (P <0 .0 5 )和 15 .38%。结论 :枳木具子水提取物在体外显示细胞毒作用 ,体内实验有抑瘤作用。

Objective:To study cytotoxic effect and antitumor effect by Hovenia dulcis Thunb(HDT).Methods:MTT assay method and in vivo antitumor test were used.Results:IC50 of HDT on human hepatoma BEL-7402 cell was 14.0mg/ml.Under the dose of 0.08,0.4,2g/kg.d(PO)×10d,the inhibitory rates of HDT on hepatoma H 22 in mice ranged 15%,37%,25%respectively.Conclusion:HDT possessed in vitro and vivo antitumor activities.