补肾法对去卵巢大鼠骨组织中TGF-β_1及Fas的影响

The effect of kidney invigoration on TGF-β_1 and Fas in Bone in Ovariectomized Rats

目的 :观察中药补肾方对去卵巢大鼠骨组织中 TGF-β1 及 Fas的影响。方法 :复制骨质疏松症大鼠模型 ,假手术组仅摘除小段肠系膜。各组动物术后常规喂养 3个月后 ,以补肾方为治疗药物 ,尼尔雌醇片为阳性对照 ,生理盐水为阴性对照 ,分别给药 3个月后处死取材。应用免疫组化 SABC法检测骨组织中的 TGF-β1 和 Fas抗原的表达情况。结果 :TGF-β1 主要在成骨细胞的胞膜上和胞浆内表达、Fas抗原主要在破骨细胞的胞浆内表达。中药组骨组织中 TGF-β1 及 Fas抗原表达的阳性颗粒的实面积、平均光度、阳性单位值较模型组明显增高 ( P<0 .0 5 ) ;而与雌激素组、假手术组比较则无明显差异 ( P>0 .0 5 )。结论 :补肾法能促进成骨细胞骨形成活性、抑制破骨细胞骨吸收活性 ,其作用机理之一可能是通过调控某些影响骨形成及骨吸收的局部细胞生长因子及凋亡基因 (如 TGF-β1 、Fas)而达到的。

Objective:To investigate the effects of kidney invigoration on TGF-β 1 and Fas in bone in ovariectomized rats. Methods:Ovariectomy was used in all of Model,TCM and Estrogen.The small segment mesentery was cut in Sham. All animal were fed in nomal ways for three months After operation. rats of TCM were given Bu Shen prescription of TCM , rats of Estrogen were given nilestriol as positive control, and rats of Model were given Saline as negative control for three months respectively. The methods of immunohistochemistry SABC were used to detect the expressions of Transforming growth factor -β 1 ( TGF-β 1) and Fas antigen in bone. Results:TGF-β 1 was expressed chiefly in osteoblastic membrane and plasm but Fas antigen was expressed chiefly in osteoclastic plasm in bone.True area,average light degree and positive unit of TCM by expressions of TGF-β 1 in osteoblast and Fas antigen in osteoclast singnificantly increased more than that of Model (P<0.05) and these data of TCM have no difference compared with Estrogen and Sham (P>0.05). Conclusion:Bu shen prescription can promate the activity of bone formation of osteoblast and depress the activity of bong absorption of osteoclast. One of the mechanism of kidney invigoration on 0steoporosis induced by ovariectomy in rats is the regulation of some local cell growth facter and apoptosis gene affecting bone formation ans bone absorption.