人成骨肉瘤细胞系转化生长因子β1成熟肽基因克隆及序列分析

Cloning and sequencing of the gene encoding hTGF-β1 mature peptide

目的 :分析人成骨肉瘤细胞系中转化生长因子 β1(TGF β1)成熟肽基因序列 ,探讨TGF β1基因突变存在的可能性及其病理机制。 方法 :提取人成骨肉瘤细胞系SOSP 96 0 7中的总RNA ,采用逆转录PCR(RT PCR)方法获得TGF β1成熟肽基因 ,经克隆后进行序列测定和分析。 结果 :发现人成骨肉瘤细胞系中存在TGF β1基因突变 ,并引起相应多肽的结构改变。 结论 :人成骨肉瘤细胞系SOSP 96 0 7中存在TGF β1基因突变 。

Objectives:To analyze the gene coding hTGF β1 mature peptide and study the TGF β1 gene mutation in osteosarcoma cell line SOSP 9607. Methods:Total RNA was extracted from human osteosarcoma cell line, and TGF β1 gene mature region was obtained by RT PCR. The fragment was sequenced and analysed after cloning into pcDNA 3.0 vector. Results:TGF β1 gene mutation was demonstrated in human osteosarcoma which leads to the alterations of the encoded protein. Conclusions:TGF β1 gene mutation may take an important role in the pathogenesis of human osteosarcoma.