四季桂愈伤组织诱导与继代培养

Study of Callus Induction Frequency and Subculture of Osmanthus fragrans var. semperflorens

为提高四季桂的愈伤组织诱导率,以四季桂为材料,对影响四季桂愈伤组织形成和继代培养的几个主要因素进行了研究.结果表明:采用四季桂花梗作外植体,在MS+BA1.5mg/L+2,4 D0.1mg/L培养基上,暗处理7d,愈伤组织质量高且诱导率可达85.71%.用2,4 D诱导四季桂愈伤组织效果较NAA显著.继代培养以0.1mg/LNAA为生长素时,褐变数显著低于2,4 D,当2ip质量浓度为1.5mg/L时褐变数最低,为15.0%.

To improve callus induction frequency,the factors affecting the callus induction frequency and subculture of Osmanthus fragrans var. semperflorens are studied.Results show that MS; the basal medium ,added with 0.1 mg/L 2,4D +1.5 mg/L BA is able to induce the footstalk callus with optional quality after one weak in dark condition and callus induction frequency up to 85.71%.2,4D is significantly effective in callus induction frequency and brown variable in subculture is significantly higher than NAA.On MS+0.1 mg/L NAA+1.5 mg/L 2ip, brown variable 15.0% is the lowest.