引物介导原位标记技术检测乳腺癌组织中HPV16和18型感染

Detection of Human Papillomavirus Types 16 and 18 Infection in Breast Cancer Tissues by Primed In Situ Labeling

探讨引物介导的原位标记技术(Primedinsitulabeling,PRINS)在检测乳腺癌组织中人乳头状瘤病毒(humanpapillomavirus,HPV)16型和18型感染的应用和了解HPV16和18型感染与人乳腺良、恶性病变之间的关系。方法:采用引物介导的原位标记(PRINS)技术和SP法免疫组化技术检测80例乳腺癌,10例乳腺导管上皮不典型性增生,20例乳腺导管内乳头状瘤,30例乳腺腺病,30例正常乳腺组织中HPV16DNA,HPV18DNA和HPV16、18E6蛋白。结果:乳腺癌组中HPV16DNA,HPV18DNAH和HPV16、18E6蛋白的阳性表达与导管上皮不典型性增生组均无显著性差异(P>0.05),但显著高于良性病变组和正常组。中、重不典型性增生组中HPV18DNA和HPV16、18E6蛋白的表达均显著高于轻度不典型性增生组(P<0.05)。癌组中HPV16,HPV18DNA双阳性为45.0%(36/80),导管上皮不典型性增生组中HPV16、18DNA双阳性为40.0%(4/10)。结论:引物介导的原位标记技术可用于乳腺癌组织中HPV16DNA和HPV18DNA的检测,HPV16和HPV18型的混合感染可能与女性乳腺癌的发生、发展密切相关。

Objective:To evaluate the applicability of Primed in situ labeling(PRINS)for the detection of human papillomavirus(HPV)types16and18DNA in breast cancer and investi-gate the eitologic relationship between HPV16and18infection and human breast cancer.Meth ods:By using PRINS technique and immunohistochemical staining,the expression of HPV16DNA,HPV18DNA and HPV16、18E6protein were observed in80cases of breast cancer,10cases of ductal epithelial atyppical hyperpplasia,20cases of intraductal papillona,30cases of ad-nosis and30cases of normal breast tissues.Results:The expression of HPV16DNA,HPV18DNA and HPV16,18E6protein in breast cancer group were significantly higher than that in benign le-sions group and normal breast tissue group,there was no significant difference in HPV16DNA,HPV18DNA and HPV16,18E6protein between breast cancer group and ductal epithelial atypical hyperpplasia groups(P>0.05)respectively.The positive expression of HPV18DNA and HPV16,18E6protein in moderate to severe ductal epithelial atyppical hyperpplasia group were higher than that in mild ductal epithelial atyppical hyperpplasia group(P<0.05)respectively.Coexspres-sion of HPV16DNA and18DNA in cancer and moderate、severe ductal epithelial atyppical hy- perppla sia groups were45.0%(36/80)and40.0%(4/10)respectively.Conclu sion:The results indi-cated that PRINS method can be used to detect HPV16DNA and HPV18DNA in breast cancer tissues.Mixted infection of both HPV16and HPV18may play an important role in tumorigenesis of human breast cancer.