中药白术对大鼠胃肌电紊乱调节作用的机制探讨

Effect of Rhizoma Atractylodis Macrocephalae on electrogastric arrhythmia in rats and its mechanisms

目的 观察白术对大鼠胃肌电紊乱的调节作用 ,同时探讨其可能机制 ,为临床应用提供依据。方法 健康成年Wistar大鼠 3 0只随机分为 3组 :①正常对照组 ;②模型组 ;③白术 +模型组。通过胃电记录仪记录并分析各组慢波频率、慢波频率变异系数及异常节率指数 ,同时对各组大鼠胃窦组织进行P物质、血管活性肠肽及一氧化氮合酶免疫组化染色。结果 模型组大鼠胃慢波频率变异系数及异常节率指数明显高于对照组 (P <0 0 1) ,应用白术水提取液后模型组大鼠胃慢波频率变异系数及异常节率指数与对照组无明显差异 (P >0 0 5 )。模型组大鼠胃窦肌间神经丛P物质的神经含量明显低于对照组 (P <0 0 1) ,血管活性肠肽 (P <0 0 5 )及一氧化氮合酶 (P <0 0 1)神经含量明显高于对照组 ,应用白术水提取液后模型组大鼠胃窦肌间神经丛P物质神经含量明显增加而血管活性肠肽、一氧化氮合酶神经含量明显减少且与对照组无明显差异 (P >0 0 5 )。结论 白术对大鼠胃肌电紊乱具有一定调节作用 ,其机制可能与胃窦肌间神经丛P物质的分布增加及血管活性肠肽。

Objective To observe the effect of Rhizome Atractylodis Macrocephalae (RAM) on electrogastric arrhythmia in rats and its mechanisms. Methods A total of 30 Wistar rats were randomly divided into 3 groups: control group, model group and RAM plus model group. The frequency of slow wave(FSW), coefficient of variation(CV) and abnormal rhythm index(ARI) were recorded and analyzed by computer. The substance P(SP), vasoactive intestinal peptide(VIP) and nitric oxide synthase(NOS) nerves in antral myenteric plexus were observed by immunohistochemical staining. Results Compared with those of the control group, the CV and ARI in model group markedly increased( P <0.01) but no significant difference was found after treatment with RAM( P >0.05). The denites of SP nerves markedly decreased( P <0.01), VIP( P <0.05) and NOS ( P <0.01) nerves markedly increased in model group, but no significant changes of the denites of SP, VIP and NOS nerves in model plus RAM group were found( P >0.05). Conclusion RAM has regulative effect on the electrogastric arrhythmia in rats and the mechanisms may be related with the increased SP nerves and decreased VIP and NOS nerves in antral myenteric plexus.