p27Kip1和Ki-67在人肾透明细胞癌中的表达及意义

Expressions of p27Kip1 protein and Ki-67 antigen in human clear cell carcinoma of kidney and its significance

目的 通过检测细胞周期素依赖激酶抑制蛋白p2 7Kip1(p2 7)和增殖细胞核抗原Ki 67在肾透明细胞癌中的表达情况 ,探讨其与临床病理指标及预后的关系。方法 采用免疫组化SP法测定 42例肾透明细胞癌及 2 0例癌旁正常肾组织中p2 7蛋白和Ki 67抗原的表达。结果 在肾透明细胞癌与癌旁正常肾组织中p2 7或Ki 67的表达均有显著性差异(P <0 .0 5 )。肾透明细胞癌的病理分级与p2 7的表达呈负相关 ,而与Ki 67抗原的表达呈正相关 (P <0 .0 1)。肾透明细胞癌中p2 7表达与Ki 67抗原表达、肿瘤有无转移呈负相关 (P <0 .0 1) ;Ki 67抗原表达与肿瘤有无转移呈正相关 (P <0 .0 1)。结论 在人肾透明细胞癌的发生、发展及转移过程中 ,由于缺乏p2 7基因持续激活的后继支撑 ,p2 7蛋白被逐渐消耗 ,Ki 67抗原表达上调 ;p2 7表达与Ki 67表达对评估肾透明细胞癌患者预后有一定价值。

Objective To study the expressions of p27Kip1 (p27) protein and Ki 67 antigen in human clear cell carcinoma of kidney and to find out the relationship between the expression levels and the clinical pathological characteristics and prognosis in cases of clear cell carcinoma of kidney. Methods The expressions of p27 protein and Ki 67 antigen in the adjacent tissues of carcinoma in 20 cases and 42 cases of clear cell carcinoma of kidney were detected by immunohistochemistry. Results The positive rates of p27 protein and Ki 67 antigen in clear cell carcinoma of kidney were significantly higher than those of the both in the adjacent tissues of carcinoma( P <0.05), and p27 and Ki 67 were not concurrently expressed in renal clear cell carcinoma(RCCC). The histological tumor grading was negatively correlated with the expression rate of p27( P <0.01) but positively with the expression of Ki 67 antigen( P <0 01). In RCCC, negative correlation was found between the expression of p27 and that of Ki 67( P < 0.01 ) and tumor metastasis( P <0.01). Conclusion p27 and Ki 67 may play an important role in the genesis, development and metastasis of human renal clear cell carcinoma, respectively. The expressions of Ki 67 and p27 may contribute to evaluating the prognosis of patients with RCCC.