慢性苯中毒患者T淋巴细胞rDNA转录活性的研究

Study of T lymphocyte rDNA transcription activity in chronic benzene poisoning patients

目的 观察慢性苯中毒患者外周血T淋巴细胞rDNA转录活性的变化 ,探讨其对慢性苯中毒人群免疫监测的意义。方法 慢性苯中毒患者 39人、恶性肿瘤患者 2 0人和健康体检者 2 2人分别取静脉血 ,通过细胞培养、银染等方法 ,并采用I CLQ细胞分析系统 ,分别测定核仁积分面积与细胞核积分面积的比值 (IA)和核仁银染积分光密度与细胞核银染积分光密度的比值 (IOD)。结果 (1 )慢性苯中毒患者IA和IOD值分别为 7.95 %± 1 .1 3 %和 7.1 5 %± 1 .1 5 % ,较正常对照组 (IA和IOD值分别为 9.59%± 1 .2 6 %和 8.92 %± 1 .1 8% )明显降低 ,差异有显著性 (P <0 .0 1 ) ;慢性中度苯中毒组IA和IOD值分别为 6 .54 %± 0 .88%和 5 .47%± 0 .80 % ,较观察对象组 (IA和IOD值分别为 7.98%±1 .0 6 %和 7.1 3 %± 0 .96 % )和轻度中毒组 (IA和IOD值分别为 8.1 9%± 1 .0 6 %和 7.44 %± 1 .0 6 % )明显下降 ,差异有显著性 (P <0 .0 5)。 (2 )恶性肿瘤组IA和IOD值分别为 4 .1 0 %± 1 .50 %和 3 .67%±1 .42 % ,较正常对照组和慢性苯中毒组均明显下降 ,差异有显著性 (P <0 .0 1 )。

Objective To observe the changes in peripheral blood T lymphocyte rDNA transcription activity and to study the significance of immune monitoring for patients with chronic benzene poisoning. Methods Venous blood samples were withdrawn from 39 patients with chronic benzene poisoning,20 patients with malignant disease and 22 healthy controls.Cell culture,argyrophil staining method,I CLQ cell image analysis system were used in this study.rDNA transcription activity which was expressed by the ratio of integrated area( IA ) of nucleolus to that of nucleus,and the ratio of integrated optical density( IOD ) of argyrophilic nucleolus to that of argyrophilic nucleus. Results (1)The value of IA and IOD in chronic benzene poisoning patients(7.95%±1.13% and 7.15% ±1.15% respectively) were lower than those in controls(9.59%±1.26% and 8.92%±1.18% respectively), P <0.01.The value of IA and IOD in chronic moderate benzene poisoning patients(6.54%±0.88%) and (5.47%±0.80%) were lower than those to be observed(7.98%±1.06% and 7.13%±0.96% respectively) as well as in mild poisoning patients(8.19%±1.06% and 7.44%±1.06% respectively), P <0.05.(2)The value of IA and IOD in malignant group(4.10%±1.50%,3.67%±1.42%) were much more lower than those in controls and in chronic benzene poisoning patients( P <0.01). Conclusion rDNA transcription activity may be an index to monitor the cellular immune function of chronic benzene poisoning patients.