Ⅰ型对氧磷酯酶基因Gln/Arg192遗传多态性与冠心病相关性的初步研究

Distribution of paraoxonase1 gene Gln/Arg192 polymorphism in patients with coronary heart disease

目的 探讨中国北方地区Ⅰ型对氧磷酯酶 (paraoxonase 1 ,PON1 )基因Gln/Arg1 92遗传多态性与冠心病发病的关系。方法 应用聚合酶链反应 (polymerasechainreaction ,PCR)及限制片段长度多态性 (restrictionfragmentlengthpolymorphisms ,RFLP)技术 ,检测 49例老年冠心病患者和 38例健康老年对照者的PON1 Gln/Arg1 92基因多态性 ,等位基因以A/B表示。结果 冠心病组与健康组比较各基因型分布差异具有显著性意义 (χ2 =6 .35 ,P =0 .0 4 2 )。B等位基因在冠心病组明显增高 (0 .56vs 0 .37)。B等位基因是中国北方地区冠心病发病的危险因素 (OR =2 .1 9,95 %CI:1 .1 9～ 4 .0 5)。结论 PON1基因Gln/Arg1 92遗传多态性与中国北方地区冠心病发病明显相关。

Objective To study the association of the human paraoxonase 1 (PON1) Gln/Arg192 polymorphism genotypes and coronary heart disease (CHD). Methods PON1 glutamine 192 (A type)/arginine (B type) polymorphism was determined using polymerase chain reaction(PCR) and restriction fragment length polymorphisms(RFLP), in 49 elder patients with CHD and 38 normal elder controls. Results There was significant difference in frequencies of genotypes between CHD and control groups(χ 2= 6.35 ,P= 0.042 ). B allele frequency of PON1 Gln/Arg192 polymorphism in CHD cases was higher than that in control group ( 0.56 vs 0.37 ). The B allele of PON1 Gln/Arg192 was proved to be a risk for developing CHD in Chinese patients of north area (OR= 2.19 ,95%CI: 1.19 ～ 4.05 ). Conclusion The Gln/Arg192 polymorphism of PON1 is obviously associated with the risk of CHD. The significant difference of PON1 Gln/Arg192 among various races was observed.