生物陶瓷骨内植入后与组织间的界面研究

Study on the interface between tissue and bioceramic implanted into bone

将β-TCP陶瓷植入大白兔的股骨内并定期注射四环素,分别在光学显微镜、荧光显微镜或扫描电子显微镜下观察新骨的形成和成骨过程,研究了β-TCP植入体内后与组织间的界面作用以及磷酸钙生物陶瓷的成骨作用。结果表明,在类骨质表面有大量的成骨细胞,间充质细胞增生和浸入。植入β-TCP陶瓷两个月后,类骨质通过钙化转变为编织骨。植入三个月后,出现由骨桥连接的“骨岛”,β-TCP陶瓷降解,并被新骨分散。植入六个月后,新的骨髓腔形成,编织骨变成板层骨。八个月后,在哈弗氏骨板上出现材料颗粒,形成典型的松质骨结构。因此,无生命的钙磷材料在体内可以参与有生命的组织活动。

The interface of porous β-TCP ceramics implanted in rabbit was investigated in order to study the osteogenesis of calcium phosphate bioceramics. β-TCP ceramics (5 mm diameter and 8 mm height) were implanted in the femur of rabbits, and tetracycline was injected termly. The samples of bone were taken 1, 2, 3, 4, 5, 6 or 8 months after operation, respectively. The new bone formation and osteogenesis process on the interface were observed by using the light microscope, fluorescent microscope, scanning electron microscope and histochemical test. The results showed that bone formation rate increased faster than that of contrast groups. The interface was visible 1 month after operation, and osteogensis was active. There were abundant osteoblasts, mesenchymal cell hyperplasia and incursion on the surface of osteoid. The osteoid turned into woven bone through calcification 2 months after operation. Bone-island appeared and connected by bone-bridge 3 months after operation. β-TCP ceramics degraded and were dispersed by new bone. New marrow cavity formed and woven bone became bone lamella 6 months after operation. The granules of material were found on the Haversian lamella 8 months after operation. The typical structure of spongy bone formed. It can be concluded that the non-living calcium-phosphate materials can participate in the activity of living tissue in vivo.