异丙酚对大鼠局灶性脑缺血/再灌注后细胞凋亡的影响

Effects of propofol on apoptosis following focal cerebral ischemia-reperfusion in rats

目的 观察异丙酚对大鼠局灶性脑缺血/再灌注后细胞凋亡的影响。方法雄性SD大鼠,采用可逆性大脑中动脉内线栓法建立局灶性脑缺血/再灌注模型。缺血2 h,再灌注2h后断头取脑,采用脱氧核苷酸末端转移酶介导的DNA原位末端标记技术检测细胞凋亡变化。HE染色,光镜观察细胞结构的改变。结果 光镜检查发现,异丙酚组细胞肿胀坏死明显减轻,异丙酚可显著减少脑缺血/再灌注后神经细胞的凋亡。结论 静脉麻醉药异丙酚具有拮抗大鼠局灶性脑缺血/再灌注损伤作用,对缺血/再灌注的神经细胞有明显的保护作用。

Objective To investigate the effects of propofol on apoptosis following focal cerebral ischemia-reperfusion in a rat model of reversible middle cerebral artery occlusion(MCAO) .Methods Twenty-four male SD rats weighing 300-500 g were randomly divided into 3 groups: group I sham operation; group Ⅱ MCAO + normal saline; group Ⅲ MCAO + propofol. MACO was produced by insertion of a 2-0 nylon filament with rounded end into internal carotid artery toward brain until resistance was encountered. The depth of insertion was about 2 mm. The filament was withdrawn after 2 h for reperfusion. In group I (sham operation) carotid artery was exposed but no filament was inserted. In group Ⅲ (propofol) intraperitoneal propofol 100 mg·kg-1 was administered 20 min before reperfusion; while in groupⅡ(normal saline) normal saline was given instead of propofol 20 min before reperfusion. At the end of 2 h reperfusion the animals were decapitated and the brain was removed. Apoptosis was identified by TUNEL technique. Cellular structure was examined under light microscope. Results Propofol significantly inhibited neuronal apoptosis following focal cerebral ischemia-reperfusion. Swelling and necrosis of the neurons were significantly attenuated in group III . Conclusion Propofol protects neurons against focal cerebral ischemia-reperfusion in rats.