局部应用地塞米松增加皮瓣成活的实验研究

Increasing survival areas of flap with topically used dexamethasone

目的 研究地塞米松增加皮瓣成活面积的作用机理。方法 2 4只豚鼠随机分成实验组和对照组两组 ,分别于实验组与对照组豚鼠的背部形成任意皮瓣。将实验组的留置硅胶管中注入地塞米松 (5mg/kg) ,每隔 36h重复等量给药 ,1 0 8h后给药量逐部减少 ,1 80h时停药。将对照组的留置硅胶管中注入生理盐水 (2ml/kg) ,1 0天时观察皮瓣成活面积 ,缝合皮瓣 2 4h后于皮瓣中央取材观察皮瓣组织学。结果 皮瓣成活率 ,实验组为 (72 .5± 4 .9) % ,对照组为 (49.2± 3 .7) %。组织学显示实验组皮瓣中性粒细胞明显少于对照组。结论 局部应用地塞米松增加皮瓣成活 ,其方法简单、有效。

Objective To study of the role dexamethasone in increasing flap survival areas. Methods 24 guinea-pigs were equally divided into experimental and control groups. Random flap were made on dorsom. 5?mg/kg dexamethsone were given through silicone duct in experimental group every other 36 hours from flap clousure, the drug dosages were decreased gradually and stopped 180 hours. 2?ml/kg normal saline was given in control group. The survived flap areas were measured 10 day postoperatively, histological changes of central flap were observed under light microscope 1 day postoperatively. Results Flap survived areas were (72.5±4.9)% and (49.2±3.7)% on experimental and control group respectively. Less neatrophil werefound in experimental groups than that in control. Conclusion Dexamethsone given topically is an effective drug to increase flap survival areas. The protection mechanisms could be correlated with its less neutrophils infiltration into flaps.