乙型肝炎病毒基因定量检测及临床应用

QUANTITATIVE DETECTION AND CLINICAL APPLICATION OF HEPATITIS B VIRAL GENE

目的 :了解乙型肝炎患者血清乙型肝炎病毒基因 (HBVDNA)水平与乙型肝炎病毒 (HBV)感染的临床关系及HBVDNA定量分析在拉米夫定治疗中的应用 ;方法 :用聚合酶链反应 (PCR)技术测定 1 0 9例HBV感染者血清中HBVDNA水平及观察 3 5例乙型肝炎患者拉米夫定治疗效果 ;结果 :1 0 9例HBV感染者血清HBVDNA水平与乙型肝炎病毒五项指标 (HBV -M)的阳性模式有一定相关性 ,在HBsAg、HBeAg阳性模式中约有93 %以上病例HBVDNA阳性 ,其含量最高 ,而在HBsAg ,抗 -HBe,抗 -HBc阳性模式中其HBVDNA阳性检出率仅为 6 7.7% ,其含量不高。HBVDNA水平与乙型肝炎临床分型的相关性不显著 (P >0 .0 5)。拉米夫定治疗前后血清HBVDNA含量有显著性差别 (P <0 .0 1 ) ;结论 :应用PCR技术定量检测血清HBVDNA含量是诊断乙型肝炎和了解病毒复制与临床关系的重要指标 。

Objective:To investigat the relationship between serum hepatitis B viral gene (HBVDNA) and the patients with hepatitis B, and application of HBVDNA quantitative detection in clinical treatment with Laminudine.Methods:The study subjects consisted of 109 patients with hepatitis B. 30 of 109 cases were only HBsAg carriers; numbers of mild, middle and severe chronic hepatitis B were 27, 28 and 24 respectively. The serum samples of all patients were collected at fasting in 109 patients with hepatitis B, and before and after treatment with laminudine (1 time/day, 100mg/time) in 35 patients with hepatitis B. The contents of HBVDNA were detected by polymerase chain response (PCR).Results:There is a correlation between level of HBVDNA and HBV mark patterns. The HBVDNA positive rate was 93% in patients with HBsAg and HBeAg positive patterns and the level of HBVDNA was higher. HBVDNA positive rate was 67% in HBsAg, anti-HBe and anti-HBc patterns. And the level of HBVDNA was lower than that of other patterns. There is a significant difference between before and after treatment with laminudine in content of HBVDNA.Conclusions:It is an important way that the level of HBVDNA was quantitatively detected by PCR for diagnosis and therapy of hepatitis B as well as relationship between HBV complication and clinical symptoms.