儿茶素对肾病大鼠肾小球脏层上皮细胞增生的影响

Effect of Catechin on Glomerular Visceral Epithelium Cells Proliferation

目的 探讨儿茶素对肾小球上皮细胞增生的影响 ,阐明儿茶素对防治肾病综合征的部分可能机制。方法 36只SD雌性大鼠随机分成正常组、肾病组、激素组、儿茶素预防组、儿茶素治疗组、儿茶素 +激素治疗组共 6组。实验末应用生化法测定2 4h尿蛋白排泄量 ,应用免疫组化法测定肾小球上皮细胞细胞周期调控蛋白P2 1、P2 7、TGF -β1、PCNA阳性表达率 ,并应用半定量评分法对各组大鼠肾小球与肾小管间质病理改变进行计量分析。结果 与肾病组相比 ,儿茶素治疗组、儿茶素预防组 ,儿茶素 +激素联合治疗组大鼠肾小球上皮细胞P2 1(P <0 0 1)、P2 7(P <0 0 1)、TGF -β1(P <0 0 5 ,0 0 5 ,0 0 1)表达降低 ,差异有显著意义 ,Cy clinD1(P <0 0 1,0 0 5 ,0 0 1)、PCNA(P <0 0 5 ,0 0 1,0 0 1)表达 ,差异有显著意义。实验末 ,2 4h尿蛋白的排泄量依次为 :肾病组 >儿茶素治疗组 >儿茶素预防组 >激素治疗组 >联合组 ,各组与肾病组大鼠相比 ,差异有显著意义。病理损害儿茶素治疗组、儿茶素预防组 ,儿茶素 +激素联合治疗组大鼠较肾病组减轻 (P <0 0 5 ,0 0 1,0 0 1)表达 ,差异有显著意义。病理积分与实验末 2 4h尿蛋白排泄及肾小球上皮细胞P2 1、P2 7、TGF -β1表达正相关 (γ =0 748,0 70 2 ,0 82 1,0 687。

Objective To investigate the effect of catechin on glomerular visceral epithelium cells proliferation, and elucidate the partial possible mechanism of catechin on the treatment of nephrotic syndrome and prevention of chronic renal injuries. Methods Thirty-six female SD rats were randomly divided into six groups , control, nephrotic, dexamethasone-treated, catechin-prevented, catechin-treated, dexamethasone plus catechin-treated groups, at the end of experiment, the excretion of 24hrs urinary protein were detected by means of biochemistry assay. The expression of related cell cycle regulatory protein such as CyclinD1,P21,P27,TGF-β1,PCNA in glomerular visceral epithelium cells were measured with the immunohistochemical technique. A semiquantitative score was used to evaluate the injury degree of glomerular and tubulointerstitium.Results Compared with nephrotic group, P21(all P<0 01),P27(all P<0 01),TGF-β1(P<0 05,0 05,0 01) expressions of glomerular visceral epithelium cells in catechin-prevented group, catechin-treated group, catechin plus dexamethasone-treated group were significantly increased, CyclinD1(P<0 01,0 05,0 01),PCNA(P<0 05,0 01,0 01) expressions of glomerular visceral epithelium cells in catechin-prevented group, catechin-treated group, catechin plus dexamethasone-treated group were significantly decreasd. The excretion of 24hrs urinary protein were that in nephrotic group> catechin-treated group > catechin-prevented group > dexamethasone-treated group > catechin plus dexamethasone-treated group. Compared with nephrotic group, the renal pathologic score were significantly different among the nephrotic group and the catechin-treated group (6 80±0 84,P<0 05), catechin-prevented group(6 50±1 00,P<0 01), dexamethasone-treated group (4 40±0 89, P<0 01), and dexamethasone plus catechin-treated group (3 40±0 55,P<0 01). The renal pathologic scores were positively correlated well with the expression of P21, P27, TGF-β1 and the excretion of 24hrs urinary protein(γ=0 748,0 702,0 821,0 687,all P<0 01), and were negatively correlated well with PCNA, CyclinD1(γ=-0 668,-0 744, all P<0 01). Conclusions Chronic progressive renal lesions could be alleviated through catechin improving glomerular visceral epithelium cells proliferation, and decreasing the excretion of 24hrs urinary protein.