摘要
目的 探讨 β型趋化因子MCP -1(monocyteschemotacticprotein -1,MCP -1)在充血性心力衰竭发病中的作用。 方法 用RT -PCR检测 2 5例充血性心力衰竭患者和 10例正常人外周血单个核细胞MCP -1的表达 ,并用Bio -Rad图像分析系统对其进行相对定量 ,相关分析法确定MCP -1与充血性心力衰竭各临床指标之间的关系。结果 心力衰竭患者MCP -1的表达水平与正常对照组相比显著上调 (P <0 0 1) ;源于高血压、冠心病和扩心病的心力衰竭患者MCP -1表达水平与对照组相比均有显著性差异(P <0 0 1) ;扩张型心肌病心力衰竭患者MCP -1的表达水平显著高于高血压性心力衰竭组 (P <0 0 5 )。MCP -1的表达水平与外周血白细胞计数呈正相关 (γ =0 473,P <0 0 5 ) ,与淋巴细胞计数呈正相关 (γ =0 42 5 ,P <0 0 5 ) ,而与心力衰竭患者的射血分数呈负相关 (γ =-0 64 8,P <0 0 5 )。结论 充血性心力衰竭患者外周血趋化因子MCP -1表达上调可能是心力衰竭发生的病理生理因素之一 。
Objective To explore the role of β chemotactic factor- MCP-1 in the pathogenesis of congestive heart failure by detecting its expression changes.Methods PBMCs were collected from 25 patients with congestive heart failure and 10 healthy controls. RT-PCR was used to detect the mRNA expression of MCP-1 in PBMCs. Correlation of the clinical indexes of heart failure patients with to their MCP-1 mRNA expression levels were analysed.Results The expression level of MCP-1 in the heart failure patients was higher significantly than that of controls (P<0 001). In the heart failure patients from hypertension, or coronary heart disease, or dilated cardiomyopathy,the MCP-1 expression level was significantly higher than that of controls (P<0 01);The MCP-1 expression level of the patients with dilated cardiomyopathy was higher significantly than that of hypertension patients (P<0 05). Correlation analysis showed that the expression level of MCP-1 was correlated with not only white blood cell count (γ=0 473,P<0 05), but also lymphocyte count (γ=0 425,P<0 05), and it was significantly inversely correlated with ejection faction (EF) as well.(γ=-0 648,P<0 05).Conclusions The up-regulation of the expression of chemotactic factor MCP-1 in PBMC may be one of the pathophysiological factors in development and attack of congestive heart failure.Its mechasism may be related to the activation of the white blood cells.
出处
《中国医师杂志》
CAS
2003年第4期495-498,共4页
Journal of Chinese Physician
