裸鼠肾包膜下移植法与四唑盐比色法检测直肠癌对抗癌药物的敏感性

Measurement of the Rectum Carcinoma's Sensitivity to the Anticancer Medicine by Nude-mouse Subrenal Capsule Assay and MTT Assay

目的 评价SRCA法与MTT比色法测定肿瘤对药物的敏感性试验中的差异 ,探讨引起这种差异的可能原因 ,为临床应用提供理论依据。方法 取新鲜直肠癌标本进行SRCA法及MTT法 ,分为 5 -FU、MMC、MTX组。SRCA法术后第 1、3、5d裸鼠各组分别皮下注射 5 -FU、MMC、MTX ,术后第 6d测定移植肿瘤消退率 ;MTT法各组以相应的药物的血浆峰浓度的 1/10作为试验浓度 ,第 4d测定肿瘤细胞的抑制率。结果 60组肿瘤对化疗药物敏感性结果中 ,符合 2 0对 ,不符合 40对 ,两方法间药物敏感性阳性和阴性结果符合率为 33 3% ,差异有统计学意义。

Objective To evaluate the difference between subrenal capsule assay (SRCA) and monotetrazolium (MTT) assay in anticancer chemosensitivity test and explore the possible cause of bringing the difference,in order to provide theoretical basis for clinical practice.Methods SRCA and MTT assay were carried out with fresh rectum carcinoma in three groups:5-FU,amethopterin(MTX) and mitomycin (MMC).The day 1,3,5 after SRCA,every subgroups was injected with 5-FU ,MMC and MTX respectively and the RR was observed at the sixth day;In MTT assay ,the drug,s concentration was 1/10 PPC of each group's drug and the IR was worked out at the fourth day after MMT assay.Results According to the result of chemosensitivity in the 60 groups,20 groups of them were accorded with,other 40 groups were not.The positive and negative according rate of tumor's sensitivity to drug was 33 3% and there was statistical difference between the two methods.Conclusions There were considerable difference between SRCA and MTT assay in anticanaer chemosensitivity test.