水飞蓟宾胶囊对异烟肼和利福平肝损害小鼠的保护作用

Protective effect of Silybin Capsules on hepatic injury induced by combining isoniazid with rifampin in mice

目的 :观察水飞蓟宾胶囊 (SC)对异烟肼 (INH)与利福平 (RFP)合用致肝损伤小鼠的保护作用。方法 :测定肝损伤小鼠在给予SC后肝指数、血清谷丙转氨酶 (ALT)的活性 ,肝匀浆中谷胱甘肽 (GSH)及丙二醛 (MDA)的含量 ,肝微粒体中细胞色素P4 5 0含量及其亚型 2E1的活性。结果 :SC可对抗INH和RFP合用引起的肝指数、血清ALT水平、肝匀浆中的MDS含量及P4 5 0、4 5 0 2E1活性的升高 ,增加GSH含量 ;病理学检查SC能明显减轻肝细胞的变性和坏死。结论 :SC对INH和RFP肝毒性的保护作用与其稳定肝细胞膜、抑制脂质过氧化反应、清除自由基。

Objective: To observe the protective effect of Silybin Capsules (SC) on mice hepatic toxic injury induced by isonicotinic acid hydrazide (INH) and rifampin (RFP) when used in combination Methods: The serum level of ALT, liver index, contents of glutathion (GSH) and malondialdehyde (MDA), activity of microsomal cytochrome P450 and its isoform P4502E1 in liver were measured. Results: SC obviously inhibited the rising of liver index, serum ALT, liver MDA and the activity of microsomal cytochrom P450 and its isoform P4502E1, and increased the liver GSH. Histopathological examination showed that Silibinin Capsules evidently alleviated the condition of the degeneration of hepatic cells and that of necrosis. Conclusion: The protective effect of SC on mice hepatic injury induced by both IIVit and RFP may be related to stabilizing the liver membrane, inhibiting the the lipid peroxidation, scavenging the free radical and decreasing the activity drug metabolizing enzyme.