电针结合磁刺激对脑缺血大鼠脑组织含水量和细胞外钙离子浓度的影响

The effects of electroacupuncture combined with transcranial magnetic stimulation on water content and extracellular calcium content of brain tissues after cerebral ischemia in rats

目的 观察电针结合磁刺激对急性脑缺血大鼠脑组织含水量和细胞外钙离子含量的影响。方法 将 30只雄性Wistar大鼠随机分成 5组 :正常组、模型组、电针组、磁刺激组和电针加磁刺激组 ,每组 6只。制作急性大脑中动脉缺血模型 ,分别施以电针、磁刺激和电针加磁刺激 ,检测脑组织含水量和局部细胞外钙离子浓度。结果 单纯电针或磁刺激均能降低脑组织含水量和遏止细胞外钙离子浓度降低 ,而 2者合用效果更优。

Objective To observe the effects of electroacupuncture (EA) combined with transcranial magnetic stimulation(TMS) on water content and extracellular calcium content of brain tissue after cerebral ischemia in rats. Methods Thirty male Wistar rats were employed and divided randomly into 5 groups: the normal group, model group, EA group, TMS group and a EA plus TMS group. After the establishment of models of acute middle cerebral artery occlusion (MCAO), the rats were treated with EA, TMS and EA plus TMS, respectively. Then the water content and extracellular calcium content of the brain tissues were measured. The data were compared and analyzed among the groups. Results EA or TMS decreased the water content and inhibit the decrease of extracellular calcium content of brain tissue after cerebral ischemia in rats and better effect could be achieved with the combined use of EA with TMS. Conclusion EA plus TMS can effectively improve the cerebral edema and adjust the extracellular calcium content after cerebral ischemia in rats.