老年心力衰竭患者血浆可溶性TRAIL和受体DR5水平的变化

Changes in the plasma soluble TRAIL and DR5 in elderly patients with congestive heart failure

目的 :观察老年充血性心力衰竭 (CHF)患者血浆可溶性 TNF相关的凋亡诱导配体 (s TRAIL)、死亡受体(s DR5 )水平与心功能状态的关系。方法 :用酶联免疫吸附法 (EL ISA)检测 4 1例老年 CHF患者和 2 0例健康老年人(对照组 )血浆 s TRAIL 和 s DR5水平 ,用多普勒超声心动图测定 CHF患者左室射血分数 (L VEF)。结果 :1CHF患者血浆 s TRAIL 水平较健康对照组升高 (1.4 5± 0 .5 2 ng· m L- 1 vs0 .93± 0 .12 ng· m L- 1 ,P>0 .0 5 ) ;CHF患者血浆 s DR5水平 (41± 8pg· m L- 1 )较对照组 (<6 pg· m L- 1 )明显升高 ,且随心功能损害程度加重而升高 ,心功能 级者显著高于 , 级 (P<0 .0 5 ,P<0 .0 1)。 2 L VEF和 s DR5水平呈显著的负相关 (r=- 0 .5 2 ,P<0 .0 1) 3高血压心脏病 CHF患者血浆 s DR5水平明显高于其它病因 CHF患者。结论 :血浆 s DR5水平可能是反映老年

AIM: To investigate the plasma level of soluble TRAIL and soluble DR5 in the elderly patients with congestive heart failure (CHF). METHODS: The levels of the plasma sTRAIL and sDR5 were determined by enzyme linked immunosorbent assay (ELISA) in 41 elderly CHF cases and 20 elderly health cases as the control. Left ventricular ejection fraction (LVEF) was measured by colour Dopper echocardiography. RESULTS: ①The levels of sTRAIL showed no significant difference between the CHF groups and the control group (1.45±0.52 ng·mL -1 and 0.93±0.12 ng·mL -1 , P >0.05); the levels of sDR5 of CHF groups (41±8 pg·mL -1 ) were significantly higher than those of the control group (<6 pg·mL -1 ) and the level of sDR5 increased significantly with the decrease of the cardical function. The plasma level of sDR5 in elderly patients of Class Ⅳ were higher than those of Classes Ⅱ and Ⅲ( P <0.05 and P <0.01, respectively). ② The plasma levels of sDR5 were negatively correlated to LVEF( r =-0.52, P <0.01). ③The levels of sDR5 were significantly higher in the cases with hypertensive heart disease. than in any other CHF cases due to other factors. CONCLUSION: The determination of sTRAIL and sDR5 may serve as a useful tool for the evaluation of the cardiac function and of the myocardial cell apoptosis.