摘要
采用一种经修改的硫酸铵沉淀及反向抽提法,从猪心肌粗提液中纯化得比活性为60 u/mg的乳酸脱氢酶(LDH),纯化倍数为50倍,酶活性回收为78％。该方法成本低,特别适用于大规模纯化乳酸脱氢酶。
A modified method of ammonium sulfate precipitation and back-extraction was used, lactate dehydrogenase (LDH) with the specific activity(60.09u/mg) has been isolated from the crude extract of porcine cardiac muscle. This procedure yields a 50-fold purification with 78％ recovery. The method is low cost and especially adaped for the large-scale LDH purification.
出处
《河北省科学院学报》
CAS
1992年第3期60-63,共4页
Journal of The Hebei Academy of Sciences
