超声心动图定量犬冬眠心肌的方法学研究

Study on Quantitative Measurement of Hibernating Myocardium with Echocardiography in Dogs

目的:建立经胸小剂量多巴酚丁胺超声心动图激发试验(Dobu-UCG)评价犬冬眠心肌定量的方法学。材料和方法:成功建立冬眠心肌犬7条3～6个月后进行DoBu-UCG试验,测量Dobu-UCG后的心肌梗死区运动幅度(wall motion amplitude of area with myocardium infarction,MMI)和非梗死区心内膜运动幅度(wall mo-tion amplititude of areas with non myocardium infarction,MNMI)的比值(MMI/MNMI),以及试验后MMI/MNMI与试验前MMI/MNMI的差值(D值);并与实验后测量组织切片的冬眠心肌面积(the area oftotal alive myocardium,ATAM)和梗死区总面积(the area of total infarction myocardium,ATIM)的比值(ATAM/ATIM)进行比较。结果:Dobu-UCG激发试验后MMI/MNMI比值、D值分别与ATAM/ATIM进行对比分析,显示两者均高度相关(r=0.83～0.90,P分别为<0.05和<0.001)。结论:测量Dobu-UCG激发试验后MMI/MNMI比值,以及试验后、前MMI/MNMI差值(D)是定量梗死区内冬眠心肌的较好方法。

Purpose: The purpose of this study was evaluated the value of quantitative measurement of experimental dog' s hibernating myocardium by excitation trial with low dose dobutamine echocardiography (Dobu -UCG) .Materials and Methods: 11 experimental myocardium infarction dogs were built, 3-6 months later, 7 alive doys from 11 were give Dobu - UCG experimental study. Results: Compared with pathological examination which was ration (ATAM/ATIM)of area of total alive myocardium (ATAM) to area of total infarction myocardium (ATIM) , and Dobu - UCG the test result displayed that there were ration (MMI/ MNMI) of wall motion amplitude of area with myocardium infarction(MMI) to wall motion amplititude of areas with non myocardium infarction(MNMI) after Dobu - UCG, and the difference (D) between MMI/ MNMI ratio before trial and after trial. The excellent correlation between MMI/ MNMI ratio after Dobu - UCG test and ATAM/ATIM ratio measured by histologically (r = 0.83 - 0.90, P < 0.05 or P < 0.001) . Conclusion: MMI/MNMI and D can quantify the hibernating myocardium from infarction myocardium after excitation trial by Dobu - UCG.