摘要
目的 :建立儿童急性呼吸道感染的PCR检测方法 ,并与常规方法进行比较。方法 :选择编码外膜蛋白P6基因作为靶片段设计引物 ,分别用PCR方法及常规培养法检测 78例急性呼吸道感染儿童。结果 :建立的PCR检测法可以检测 10～ 5 0个流感嗜血杆菌 ;78例急性呼吸道感染儿童中PCR方法检出 4 2株 (5 3.8% ) ,常规培养方法检出 33株 (4 2 .3% ) ,培养法检测阳性者PCR方法均为阳性。PCR方法较培养法有显著性差异。结论 :PCR方法检测呼吸道流感嗜血杆菌感染比常规方法更快速 ,更简便 ,更敏感。PCR方法检测流感嗜血杆菌有可能成为临床流感嗜血杆菌感染诊断的一种实用、理想的方法。
Objective:To develop a PCR method for detection of Haemophilus influenzae in children being diagnosed Acute respiratory infection, and compare the results with those obtained by the culture method. Methods: using the the primer set which was selected from the nucleotiede sequence encoding the outer-membrane protein P6, we applied the PCR method in dectecting Haemophilus influenzae in 76 children with Acute respiratory infection. We also detected the children by culture method .Results:The PCR method described had enough sensitvity to detect 10～50 Haemophilus influenzae cells. Haemophilus influenzae was checlced on 33 samples of 76 ARI children (42.3%) by culture method,while 42 samples (53.8%) were by P6-positive with PCR All samples.incohich Haemophilus influenzae was cultured were detected negative by PCR while they were positive by the cultured method.There isasignificant difference between PCR and the culture method (P<0.01). Conclusion: PCR method is faster ,EASIER and more sensitive than the cultured method in the detection of Acute respiratory infection . it is likely to become a practical and alternative approach to detecting Haemophilus influenzae by PCR. [
