血管内皮生长因子在肺癌组织中表达的研究

A study on vascular endothelial growth factor expression in lung carcinoma

目的 观察血管内皮生长因子 (VEGF)表达、肺癌组织微血管密度 (MVD)和肺癌临床病理指标及预后的关系 ,探讨VEGF的作用机制。 方法 应用免疫组化方法检测 6 0例肺癌患者肺癌组织标本MVD及VEGF的表达。 结果 ①VEGF在肿瘤细胞、肺癌组织内巨噬细胞呈阳性 ,部分血管内皮细胞及成纤维细胞也呈弱阳性。②肺癌组织MVD计数在肺癌各项临床病理指标中无显著性差异 ,P >0 0 5 ;VEGF表达在不同N分期 (淋巴结转移 )及临床分期之间存在显著性差异 ( P <0 0 1,P <0 0 5 ) ,并随淋巴结转移及临床分期的进展而有逐渐增强的趋势。③血管高密度组VEGF表达阳性率为 77 4 %,明显高于血管低密度组 ( 44 8%) ,P <0 0 1。④CoxRegression风险比例模型分析 ,肺癌临床分期及VEGF表达可作为判断肺癌患者预后的独立指标。 结论 ①VEGF可由肿瘤细胞、巨噬细胞及部分血管内皮细胞及成纤维细胞等多种细胞产生 ,通过旁分泌作用促进肿瘤血管生成。②VEGF在肺癌淋巴结转移及临床分期中起重要作用 ,并可作为判断肺癌患者预后的参考指标之一。

Objective To investigate expression vascular endothelial growth factor(VEGF) and its relation with intratumoral microvascular density(MVD),clinical features and prognosis of lung cancer. Methods Surgical samples of 60 patients with primary lung cancer were measured.Immunohistochemisty on paraffin sections was performed with anti-human factor Ⅷ antibody to study the MVD,and with antibody to VEGF to investigate the expression of VEGF in tissue of lung cancer. Results ①Expression of VEGF in tumor cells,macrophage,endothelial cells and fibroblasts was demonstrated.②There was no significant difference between low MVD and high MVD groups in clinical features,but there was significant difference in expression of VEGF of patients with different lymphatic metastasis and clinical stage.③The expression of VEGF in high MVD group was higher than that in low MVD group,P<0.01.④By COX's proportional hazard model analysis,the expression of VEGF was considered an independent predictor for the prognosis of lung carcinoma. Conclusion ①VEGF appears to be produced by tumor cells,macrophage,endothelial cells and fibroblasts,may promote tumor angiogenesis by an aparacrine loop.②VEGF plays an important role in lymphatic metastasis and is a reliable prognostic parameter in lung cancer.