高血脂血瘀模型白细胞粘附活化和白细胞介素2、6和8的变化

Effect of Activation Leukocyte and Change of Leucocyte Medium on Hyperlipidemia and Blood Stasis Animal Model Induced by Immune Damaging and Stress

建立高脂、免疫损伤、应激等综合因素所致高血脂血瘀动物模型 ,探讨动脉粥样硬化相关免疫损伤和炎症反应机制。将 4 0只Wistar大鼠随机分成高脂组、免疫损伤高脂组 (异体血清蛋白和高脂 )、免疫损伤应激高脂组(高脂、异体血清蛋白和肾上腺素 )和应激组 (单纯肾上腺素 )。观察不同模型脂质代谢、白细胞粘附活化及白细胞介质的变化。结果发现 ,和应激组比较 ,高脂组、免疫损伤高脂组和免疫损伤应激高脂组总胆固醇、低密度脂蛋白胆固醇水平明显升高 ,免疫损伤高脂组和免疫损伤应激高脂组脂蛋白 (a)水平明显升高 ,尤其是免疫损伤应激高脂组甘油三酯水平升高更显著 (P <0 .0 5和P <0 .0 1) ,高脂组、免疫损伤高脂组和免疫损伤应激高脂组白细胞介素 6水平明显升高 ,高脂组和免疫损伤高脂组白细胞介素 8水平明显升高 (P <0 .0 1) ;应激组白细胞粘附分子CD11/CD18表达率明显高于其他三组 (P <0 .0 5 ) ;各组间白细胞介素 2水平无显著差异。结果提示 ,高脂、免疫损伤、应激等综合因素所致高血脂血瘀模型 ,为中医药多环节干预动脉粥样硬化和血瘀证的作用研究提供了一个较为可靠的模型 ,同时初步阐明了免疫损伤、炎症反应机制在动脉粥样硬化发病机理中的重要作用 。

Aim To establish a new animal model which is more suitable for the study on atherosclerosis and to investigate its mechanisms and to study the immunological and inflammtional mechanisms in the pathogenesis of atherosclerosis. Methods 40 Wistar rats were randomly divided into 4 groups. Rats of the first group (n=10) were fed with high cholesterol diet (HCD). Rats of the second group (n=10) were fed with high cholesterol diet and injected with xenoma serum protein (HCD XSP). Rats of the third group (n=10) were fed with high cholesterol diet and injected with xenoma serum protein and adrenine (HCD XSP A), rats of the forth group (n=10) were fed with normal diet and just injected with adrenine (ND A). The changes of platelet function, lipid metabolism, leucocyte activation and adhesiveness, mediators of inflammation and blood vessel endothelial function were observed in four groups. Results In comparison with the ND A group, TC and LDLC of the HCD XSP A group rised significantly (P<0.01). As to the interleukins, the three groups fed with high cholesterol diet had high level of IL 6 and the HCD and HCD XSP groups had high level of IL 8, compared with the ND A group (P<0.01). But in all of the four groups, IL 2 did not have marked difference. The ND A group had the high leukocyte adhesion molecule express rate (CD11/CD18) than the other three groups (P<0.05). Conclutions The hyperlipidemia model induced by combined facters (HCD XSP A) is more suitable for the study on atherosclerosis and its mechanisms of traditional Chinese medicine treatment. And the immunological and inflammtional mechanisms play an important role in the pathogenesis of atherosclerosis.