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苦瓜蛋白对柯萨奇B3病毒性心肌炎小鼠半胱天冬酶3活性及凋亡的抑制作用 被引量:4

Inhibitory Effects of Momordicin on the Activity of Caspase 3 and Apoptosis of Myocardium in BALB/C Mice with Coxsackievirus B3 Myocarditis
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摘要 为研究苦瓜蛋白对凋亡的关键酶半胱天冬酶 3及凋亡的调节作用 ,用已经建立起来的方法从苦瓜果肉中提取苦瓜蛋白。将BALB/C小鼠分为 4组 :病毒对照组、正常对照组、药物对照组和药物治疗组 ,分别在第 0天、第 3天、第 7天和第 14天各处死 5只小鼠 ,第 2 1天全部处死动物 ,心肌组织半胱天冬酶 3活性测定按照CAL BIOCHEM公司的试剂说明书进行 ,并稍加改正 ,凋亡鉴定按照Oncogene公司的TdT DNA裂解片断末端原位标记试剂说明书进行。结果发现 ,①病毒对照组第 7天开始出现半胱天冬酶 3活性 (0 .6 3± 0 .2 1pmol/min ,n =5 ) ,第 14天的活性 (10 .9± 1.5pmol/min ,n =5 )显著高于第 7天 ,第 2 1天的活性 (12 .6± 1.3pmol/min ,n =5 )又高于第 14天。②药物治疗组 ,只有一个第 2 1天的标本有半胱天冬酶 3活性 (0 .4 1pmol/min) ,其它两组均未检测到此酶的活性。③DNA裂解片断末端原位标记法发现 ,病毒对照组在第 7天心肌中有少数细胞凋亡 ,第 14天、第 2 1天凋亡细胞明显增多 ,在非病变区域可发现单个凋亡的心肌细胞。治疗组未发现凋亡细胞 ,其它两组也未发现凋亡细胞。结果提示 ,CVB3病毒性心肌炎中发现有明显的凋亡现象 ,凋亡和半胱天冬酶 3发现于第 7天 ,且随着时间增加而逐渐加重(增强 ) ;苦瓜蛋白可显? Aim To measurement the inhibitory effect of Momordicin on the activity of caspase 3 and apoptosis of myocardium in BALB/C mice with coxsackievirus B3 myocarditis. Method Momordicin was purificated from Momordica charantia L by our previous reported methods. Four group animals were used in this experiment, such as disease group (DG), normal group (NG), Momordicin group (MG), and Momordicin therapy group (MTG). Five animals were killed at the 3rd,the 7th,and the 14th days in each group, and all were killed at the 21st day. According to the method of Calbiochem Company, the activity of caspase 3 from the heart of BALB/C mice was measured, and apoptosis was measured by terminal transferase mediated DNA nick end labeling assay (TUNEL, the method provided by Oncogene Company). Results ①The activity of caspase 3 were measurable at the 7th day in the DG (0.63±0.21 pmol/min, n=5). The activity of caspase 3 at the 14th day (10.9±1.5 pmol/min, n=5) and the 21st day (12.6±1.3 pmol/min, n=5) are higer than the 7th day, and the activity of the 21st was higher than 14th day (P<0.05). ②Only a animal has the activity of caspase 3 at the 21st day (0.41 pmol/min) in the HTG, no caspase 3 activity were detected in the animals of MG and NG. ③Only a few apoptotic cells were found at the 7th day in the DG, and more apoptotic cells were found among cardiac muscles at 14th and 21th days. Single apoptotic cardiomyocytes were also found at the outside of the pathological change areas, and no apoptotic cells were found in the animals of NG, MG, and MTG. Conclusions Distinct apoptosis were found in CVB3 viral myocarditis; the activitys of caspase 3 and apoptosis were found at the 7th day, and higher after the 7th day, single apoptotic cardiomyocytes were also found. The high dose Momordicin has distinct inhibitory effect on the activity of caspase 3 and apoptosis.
出处 《中国动脉硬化杂志》 CAS CSCD 2003年第2期107-110,共4页 Chinese Journal of Arteriosclerosis
基金 国家自然科学基金 (30 0 70 934)资助
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参考文献20

  • 1王佐,李双杰,杨永宗.苦瓜蛋白对BALB/C小鼠柯萨奇B3病毒性心肌炎核因子κB的调节作用[J].中国动脉硬化杂志,2002,10(6):479-482. 被引量:16
  • 2Chow LH, Gauntt CJ, McManus BM. Differential effects of myocarditic variants of coxsackievinus B3 in inbred mice. A pathologic characterization of heart tissue damage. Lab Invest , 1991, 64 (1) : 55-64.
  • 3Bowles NE, Towbin JA. Molecular aspects of myocanlitis. Curr Opin Cardiol, 1998, 13 (3): 179-184.
  • 4Yamamoto H, Kishimoto T, Minamoto S. NF-κB activation in CD27 singnaling:involvement of TNF receptor-associated factors in its signaling and identification of functional region of CD27. J Immunol, 1998, 161 (9): 4 753-759.
  • 5Alter P, Jobmann M, Meyer E, Pankuweit S, Maisch B. Apoptosis in myocarditis and dilated cardiomyopathy: dose enterovirus genome persistence protect from apoptosis from apoptosis? An endomyocardial biopsy study. Cardiovasc Pathol,2001, 10(5): 229-234.
  • 6Kyto V, Saraste A, Fohlman J, Ilback NG, Harvala H,. Vuorinen T, Hyypia T. Cardiomyocyte Apoptosis after antiviral WIN 54954 treatment in murine coxsackievirus B3 myocarditis. Scand Cardiovasc J, 2002, 36 (3) : 187-192.
  • 7Thomberry NA, Lazebnik Y. Caspase: enemies within. Science, 1998, 281:1 312-316.
  • 8Miura M, Zhu H, Rotell R, Hartwieg EA, Yuan J. Induction of apoptosis in fibroblasts by IL-I beta-converting enzyme, amammalian homolog of the C. elegans cell death gene ced-3. Cell, 1993, 75 (4) : 653-650.
  • 9Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activited Dnase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature, 1998, 391:43-52.
  • 10Razvi ES, Welsh RM. Apoptosis in viral infection. Adv Virus Res, 1995,45:1-60.

二级参考文献10

  • 1江苏新医学院编.中药大词典(上册)[M].上海:上海科学技术出版社,1986.1462.
  • 2Caamano J,Hunter CA. NF-kappa B family of transcription factors: central regulators of innate and adaptive immune functions. Clin Microbiol Rev,2002,15 (3): 414-429
  • 3Bottex-Gauthier C,Pollet S,Favier A,et al. The Rel/NF-kappa-B transcription factors: complex role in cell regulation. Pathol Biol (Paris),2002,50 (3): 204-211
  • 4Zhang JS,Feng WG,Li CL,et al. NF-κB regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal marcrophages:roles of PKC,PKA,ERK,p38 MAPK,and proteasome. Cell Immunol,2000,204: 38-45
  • 5Matsumori A,Kawai C,Sawada S,Yamamoto K. Experimental coxsackievirus B3 perimyocarditis in the right ventricle in BALB/c mice: a one-year follow-up study. Jpn Circ J,1980,44 (10): 842-847
  • 6Matsumori A,Kawai C. Coxsackie virus B3 perimyocarditis in BALB/c mice: experimental model of chronic perimyocarditis in the right ventricle. J Pathol,1980,131 (2): 97-106
  • 7Francosis Deryckere,Frank Gannon. A One-hour miniperparation technique for extraction of DNA-binding proteins from animal tissues. BioTechniques,1994,16 (3): 405
  • 8王佐,李全忠,杨向东,肖尚志,杨永宗.高效液相色谱分析氧化型低密度脂蛋白处理的U937细胞胞内胆固醇及胆固醇酯[J].中国动脉硬化杂志,1998,6(4):317-320. 被引量:43
  • 9李双杰,张宝林,邓晖,王佐,李湘斌,危当恒.苦瓜蛋白体外抗柯萨奇B_3病毒感染[J].湖南医科大学学报,1999,24(6):583-584. 被引量:14
  • 10孙强明.病毒与细胞凋亡[J].国外医学(病毒学分册),2001,8(3):78-81. 被引量:5

共引文献15

同被引文献45

  • 1黎锦城.苦瓜的抗炎作用[J].中药材,2001,24(7):508-509. 被引量:11
  • 2Elifuraha Francis Mchomvu,杨作成.基质金属蛋白酶及其在心肌纤维化中的作用(英文)[J].湖南师范大学学报(医学版),2006,3(3):75-81. 被引量:4
  • 3宋晓东,王仑,刘玉清,辛颖,惠汝太.柯萨奇病毒致病毒性心肌炎的细胞凋亡研究[J].病毒学报,2005,21(2):142-144. 被引量:3
  • 4吴铁吉.病毒性心肌炎诊断标准(修订草案)[J].中华儿科杂志,2000,38(2):75-75.
  • 5HimeShigekazuN. Apoptosis by death factor[ J ]. Cell, 1997,88 :357.
  • 6Cummins B, Acukland M, Cummins P. Cardiac-specific troponin Ⅰ, radioimmunoassay in the diagnosis of acute myocardial infarction. Am Heart J , 1987, 113(6): 1333-344.
  • 7Rezkalla S, Kloner RA, Khatib G, Smith F, Khatib R. Effect of metoprlol on the acute phase of Coxsackiievirus B3 murine myocarditis. J Am Coll Cardiol,1988, 12(8): 412-414.
  • 8Gauntt C, Huber S. Coxsackievirus experimental heart disease. Front Biosci,2003, 8(1): 23-35.
  • 9Luppi P, Rudert W, Licata A, Riboni S, Betters D, Cotrufo M, Frati G, Condorelli G, Trucco M. Expansion of specific alphabeta( + ) T-cell subsets in the myocardium of pstients with myocarditis and idiopathic dilated cardiomyopathy associated with Coxsackievirus B infection. Hum Immunol, 2003, 64(2): 194-210.
  • 10Caforio AL, Mahon NJ, Tona F, McKema WJ. Circulating cardiac autoantibodies in dilated cardiomyopathy and myocarditis: pathogenetic and clinical significance. Eur J Heart Fail, 2002, 4(4): 411-417.

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