白细胞精子症病人精浆活性氧与细胞因子检测及评价

Measurement of the Reactive Oxygen Species and Cytokines in the Seminal Plasma of Leukocytospermic Patients

目的 :研究不育症病人精浆中活性氧 (ROS)、超氧化物歧化酶 (SOD)及白介素 8(IL 8)的水平及其之间的关系。 方法 :收集 15例生育男性 (生育组 )、16例非感染性不育症者 (非感染组 )及 11例白细胞精子症者 (感染组 )的精液 ,进行常规精液分析 ,检测其精浆中IL 8、丙二醛 (MDA)、SOD及白细胞 (WBC)含量 ,并进行相关分析。 结果 :感染组与另两组相比 ,MDA、WBC及IL 8明显升高 (P <0 .0 0 1)。相关分析发现 ,IL 8与MDA显著相关 (r =0 .85 2 ,P<0 .0 0 1) ;WBC与IL 8间显著相关 (r=0 .818,P <0 .0 1)。 结论 :感染性不育症病人精液中 ,ROS与IL 8的增加有关 ,抗氧化系统缺陷是ROS增加的原因之一。

Objectives: To detect the levels of reactive oxygen species (ROS), superoxide dismutase(SOD) and interleukin 8(IL 8) in seminal plasma of infertile patients, and evaluate the possible relationship between those levels. Methods: Semen was collected from normal donors (15 cases), infertile men without infection (16 cases), and infertile men with infection (leukocytospermia, 11 cases). The routine analysis of semen was accomplished, and then the levels of IL 8, malondialdehyde (MDA), SOD, and white blood cell (WBC) were examined. The correlative analysis between the level of ROS and other parameters in these populations was made. Results: In leukocytospermic group, the levels of MDA, WBC, and IL 8 were higher than those in the other two groups ( P < 0.001 ). Significantly positive correlation was observed between IL 8 and MDA ( r =0.852, P < 0.001 )and between the levels of IL 8 and WBC.Conclusions: These findings suggest that increased oxidative stress in patients with leukocytospermia may cause the increase of IL 8 ( r=0.818, P < 0.01 ). The increased oxidative stress may be due to defect in ROS scavenging system.