肝内HBVDNA与血清HBV复制标志及肝细胞坏死关系的探讨

The implication of cytoplasmic HBV-DNA:HBV replication and hepatic cell necrosis

目的分析探讨HBVDNA在肝内的表达与肝细胞坏死的关系。方法采用原位分子杂交的方法检测了肝炎患者肝细胞内HBVDNA,辣根过氧化物酶—抗辣根过氧化物酶(PAP)法检测肝内HB sAg、HBcAg。结果胞浆型HBVDNA与HBV复制标志及肝细胞坏死密切相关 ;肝内HBVDNA检出率显著高于HBV非复制组(81 %) ,且往往与肝内HBsAg、HBcAg阳性部位对应 ,其分布与肝细胞坏死灶一致 ,多位于肝细胞坏死灶中间或毗邻。

Objective To investigate the correlation between intrahepatic HBVDNA and hepatic cell necrosis.Methods The in situ hybridization was introduced to visualize the presence of intrahepatic HBVDNA and the PAP assay was employed to detect the expression of HBsAg and HBcAg in the liver tissue.Results The prevalence of intrahepatic HBVDNA was higher(81%)in the group with HBV replication.The positive HBVDNA was located in the plasma of hepatocytes and distributed as foci in the liver tissue, relating to the location of HBsAg and HBcAg expression.The HBVDNA usually exists in the plasma of hepatocytes appearing in the area with focal necrosis or piece_meal necrosis and its surrounding tissue.The hydropic degeneration of different degree and pyknosis in the cells with cytoplasmic HBVDNA was also found.Conclusion It suggests that the active replication may be the major responsibility for persistant necrosis of hepatocytes.