摘要
目的 :探讨大鼠青春期和成年期糖尿病时性腺 5α 还原酶Ⅱ型的活性变化。 方法 :取 4 0d和 90d龄雄性Wistar大鼠各 30只 ,分别分为对照组 (C)、糖尿病组 (D)和胰岛素治疗糖尿病组 (ID)。采用薄层层析法测定各组附睾头、附睾尾、前列腺和睾丸组织内 5α 还原酶 Ⅱ型的活性。 结果 :①青春期大鼠性腺的各部位内 ,5α 还原酶 Ⅱ 型的活性D组均明显低于C组 (P <0 .0 1) ,而ID组明显高于D组 (P <0 .0 1) ;②成年期大鼠性腺的各部位内 ,5α 还原酶 Ⅱ型的活性在C、D和ID组各组间差异无显著性 (P均 >0 .0 5 )。 结论 :青春期大鼠性腺内 5α 还原酶 Ⅱ型的活性更易受代谢环境、激素水平及局部特异性因素的影响 ,而成年期大鼠性腺内 5α 还原酶Ⅱ
Objectives: To study the changes of sexual gland 5α reductase type Ⅱ activity in pubertal and adult rats with diabetes.Methods: We selected 40 and 90 days old male Wistar rats as pubertal and adult animal model respectively, 30 rats in each group. The rats were randomly divided into three groups: control group (C), diabetic group (D) and diabetes with insulin replacement group (ID). The activity of 5 α reductase type Ⅱ was measured with thin layer chromatography in the epididymis, prostate and testis. Results: ① In all sexual glands of pubertal rats, the activity of 5α reductase type Ⅱ in D group is significantly lower than that in C and ID groups. ② In all sexual glands of adult rats, there is no difference in the activity of 5α reductase type Ⅱ among these groups. Conclusions: The activity of 5α reductase type Ⅱ is likely to be influenced by metabolic environment, hormonal levels and local specific factors in pubertal rats, but it is relatively stable in adult rats.
出处
《中华男科学杂志》
CAS
CSCD
2003年第2期82-84,共3页
National Journal of Andrology
基金
国家自然科学基金资助 ( 3 9870 3 13 )
