纳洛酮对重型颅脑损伤大鼠的脑保护机制

The study of naloxone on treating severely traumatic brain injury in rat

目的颅脑损伤后血浆内啡肽(β-EP)、内皮素(ET)增高可加重继发性颅脑损伤,监视纳洛酮对实验性颅脑损伤大鼠β-EP、ET及脑水肿变化的影响,对探讨其治疗机制及临床应用有一定意义。方法Wister大鼠63只,随机分为实验组、对照组、空白组,自由落体法制作重型颅脑损伤模型。伤后半小时实验组大鼠腹腔注射纳洛酮(苏诺)10mg/kg,对照组注射生理盐水,分别于伤后1,3,8,24h处死,以干湿重法检测脑水肿的变化,放免法监测血浆β-EP、ET改变。结果实验组脑水肿程度较对照组轻,血浆β-EP、ET轻度升高,与对照组比有统计学差异。结论纳洛酮可以降低实验型重型颅脑损伤大鼠血浆β-EP、ET的浓度,减轻脑水肿,起到脑保护的作用。

Aim To investigate the effect of naloxon e on the change of ETβ-EP in the plasma of rat following TBI .Methods 63Wistar rats were divided into control group,experim ental group and an empty group.Referring to the free-falling-body-crash div ice of feenly .The brain injury model was es-tablished.The experimental group w ere injected with naloxone transpeniponeally by 10mg per kilog ram after half an hour,meanwhile ,th e control group were injected the same amount of normal saline.Every sub-g roup was killed at the point of 1,3,8and 24h after injury respectively,The change of brain edema were measured with wet -dry weight ,the ET,β-EP in plasma were measured by EIA .Results The degree of brain edema of experime ntal group declined,the content ofβ-EP Et in plasma increased mildly,th ere was a significant statistical difference between the experienmental group a nd the control group .Conclusion Naloxone may significantly deter th e rise in the content ofβ-EP,ET in plasma and prevent the deve lopment of main edema.