血清sICAM-1测定在判定乙型肝炎不同临床类型中的意义

Significance of serum sICAM-1 in the clinical types of hepatitis B

目的 :观察血清sICAM - 1水平在HBV感染者不同临床类型中的变化及其意义 .方法 :采用双抗体夹心ABC -ELISA法 ,对 74例不同临床类型HBV感染者及 10例正常对照者血清中可溶性细胞间粘附分子 - 1(sICAM 1)进行了定量测定 .结果 :急性肝炎 13例血清中sICAM - 1含量 (680± 2 4 8)μg·L-1明显高于正常对照组 (2 0 4± 4 6) μg·L-1(P <0 0 1) ;4 6例慢性肝炎患者血清中sICAM - 1含量 (675± 3 11) μg·L-1明显高于正常对照组 (P <0 0 1) ;其中慢性肝炎轻度 19例 ,sICAM - 1含量 (445± 12 0 ) μg·L-1;慢性肝炎中度 18例 ,sICAM - 1含量为 (63 4± 182 ) μg·L-1,明显高于慢性轻度组 (P<0 0 1) ;慢性肝炎重度 9例 ,sICAM 1含量 (93 1± 2 80 ) μg·L-1,明显高于慢性轻度组 (P <0 0 1) .5例无症状携带者sICAM 1含量 (2 12± 72 ) μg·L-1,与正常对照组差别无显著性 (P >0 0 5 ) ;10例自然恢复组含量为 (2 4 3± 86) μg·L-1,与正常对照组差别无显著性 .血清中sICAM 1含量与ALT含量呈明显正相关 ;而E抗原阳性组与E抗原阴性组差异无显著性 .结论 :血清中sICAM - 1水平测定可作为判别乙型肝炎临床类型的指标 ;sICAM 1含量与肝脏损伤程度成正比 ,sICAM

AIM: To observe the changes and significance of serum sICAM 1 in the assessing clinical types of Hepatitis B. METHODS: Double antibody sandwich ABC ELISA was employed for the quantitative measurement of serum sICAM 1 of 74 cases of different clinical types and 10 cases of normal control . RESULTS: Both the sICAM 1 level of 13 cases of acute hepatitis B (680±248) μg·L -1 and that of 46 cases of chronic hepatitis B (675±311) μg·L -1 were significantly higher than that of normal control (204±45) μg· L -1 . The sICAM 1 level of 18 cases of moderate chronic hepatitis B (634±182) μg·L -1 was significantly higher than the 19 cases of mild chronic hepatitis B (445±120) μg· L -1 . The sICAM 1 level of 9 cases of serious chronic hepatitis B (931±280) μg·L -1 was significantly higher than that of the group of mild chronic hepatitis B. The sICAM 1 level of 10 cases of completely recovered cases ( 243± 86) μg·L -1 and that of 5 cases of asymptomtic carriers ( 212± 72) μg·L -1 were not significantly different from that of the normal control. Serum concentrations of sICAM1 were significantly positively correlated with the concentrations of ALT. There was no significant difference between the HBeAg positive group and HBeAg negative group. CONCLUSION: Detection of sICAM 1 can be used as a indicator in assessing clinical types. The concentration of sICAM 1 is positivley correlated with the level of liver damage but is not related to the virus duplication.