高压氧对弥漫性脑损伤大鼠组织病理学的影响

Effect of hyperbaric oxygen on diffuse injured rats brain histopathology

目的 :研究高压氧 (HBO)治疗对弥漫性脑损伤大鼠组织病理学的影响 .方法 :采用Marmarou介绍的落体打击装置制作弥漫性脑损伤大鼠模型 ,观察大鼠脑外伤后 6h ,1d,2d ,3d ,1wk ,2wk ,3wk的病理学变化及 0 .2MPa高压氧治疗对其影响 ,用免疫组化的方法观察损伤脑区NF表达情况 ,用干湿重法测量脑组织含水量 .结果 :大鼠脑外伤后顶叶皮层与海马出现不同程度神经元变性坏死 ;脑组织含水量较正常组高 ;视束处NF染色可见神经丝蛋白凝集成块 ,周围有空隙 ;给予高压氧治疗后坏死神经元较未治疗组明显减少 ,脑组织含水量明显降低 (P <0 .0 5 ) .结论 :大鼠脑外伤后出现广泛神经元变性坏死 ,大面积水肿 ,弥漫性轴突断裂 ,而高压氧治疗可明显减轻血管源性脑水肿 ,改善脑组织供氧 。

AIM: To investigate the effect of hyperbaric oxygen on diffuse injured rats brain histopathology. METHODS: We produced head injured animal model by an weight dropt device designed by Marmarou, the effects of 0 2 MPa hyperbaric oxygen on brain injury were observed at different time after trauma(6 h, 1 d, 2 d, 3 d, 1 wk, 2 wk and 3 wk). The expression of neurofilament(NF)was observed after trauma using immunohistochemistry methods. The content of water in the rat brain tissue was measured by dried/wet weight method. RESULTS: After trauma, diffuse neuronal degeneration and necrosis were seen and brain water content increased. Axonal rupture was observed by NF staining. But the number of damaged neurons and the brain water content decreased significantly in the HBO group ( P <0 05). CONCLUSION: These results show that diffuse degeneration, necrosis, edema and axonal injury occur after trauma and HBO treatment may decrease the damaged neurons and alleviate brain edema.