基膜粘连蛋白和IV型胶原对培养脊髓神经细胞突起生长的作用

Effects of laminin and type IV collagen on the axons growth of the cultured spinal neuron

目的 :观察基膜粘连蛋白和Ⅳ型胶原在体外培养条件下对脊髓神经细胞突起生长的作用 .方法 :用基膜粘连蛋白和Ⅳ型胶原包被培养板 ,以结合了相应抗体的培养板及包被I型胶原和多聚赖氨酸的培养板为对照 ,将培养的脊髓细胞以相同浓度加入各组培养板内 .培养 5d后将各组细胞进行神经元特异性烯醇化酶 (NSE)免疫组化染色 ,利用计算机图像分析技术测定各组神经元突起的长度并进行统计学分析 .结果 :免疫组化染色可见各组均有较多的神经细胞及其突起生长 ,统计分析结果表明基膜粘连蛋白组与Ⅳ型胶原组间神经元突起生长长度无显著差异 ,基膜粘连蛋白组神经元突起生长长度长于其它各组 .Ⅳ型胶原组神经元轴突生长长度与I型胶原组间无显著差异 ,与多聚赖氨酸组有显著性差异 .基膜粘连蛋白组和Ⅳ型胶原组神经元轴突生长情况优于相应的抗体组 .结论

AIM:To detect the influence of laminin and type IV collagen on the axon growth of the cultured spinal neuron. METHODS: Laminin and collagen IV were used in the experimental group and laminin and collagen IV combined with associated antibody, collagen I and polylysine were used in the control groups. Spinal neuron cells of the same density were cultured in each group. Each group was stained by the immunochemical method of Neuron-specific Enolase (NSE) and the length of the neuron axons was measured by the computer image system. RESULTS: The spinal neuron cells and their axons were stained by NSE. The axons length of the laminin and collagen IV groups was longer than that of other groups but no significant difference was observed. CONCLUSION: Laminin and collagen IV can stimulate the axon growth of spinal neuron cells.