口服供者脾细胞诱导成年大鼠皮肤移植物存活期延长

Prolongation of survival of skin allografts in adult rat by feeding of donor spleen cells

目的 :观测口服供者脾细胞后受者大鼠皮肤移植物的存活时间 ,并探讨口服耐受的形成机制 .方法 :以纯系SD大鼠为供者 ,纯系Wistar大鼠为受者 ,行异体皮肤移植 ,将 2 0只受者大鼠随机分成 2组 :A组为对照组 ,单纯作皮肤移植 ;B组为口服抗原组 ,本组于皮肤移植前 7d始每日接受供者脾细胞 5× 10 7个 ,导管灌胃 ,7d后观察体外混合淋巴细胞培养(MLC)、体内的迟发型超敏反应 (DTH) ,并行皮肤移植 ,观察移植皮肤的存活时间 .结果 :口服抗原后 ,体外混合淋巴细胞培养及体内的DTH反应均出现明显的抗原反应性降低 ,口服抗原组的移植皮肤存活时间达到 (15 8± 1 3 )d ,而对照组为(4 9± 0 1)d ,两组差异显著 (P <0 0 1,n =10 ) .结论 :口服抗原可以引起受者对异基因抗原的特异性免疫反应降低 。

AIM: To investigate the survival time of skin allograft in recipient rat by orally feeding donors spleen cells and to study the mechanism of oral tolerance. METHODS:The inbred SD rats were chosen as skin donors and inbred Wistar rats as recipients, and heterotopic skin transplantations were performed. The 20 recipients were classified into 2 groups randomly. In group A (control group) heterotopic skin transplantations were performed and in group B (feeding group) heterotopic skin transplantations were performed following a 7 day feeding of 5×10 7 (daily) donor splenocytes by means of gastric intubation. The mixed lymphocyte culture in vitro and delayed type hypersensitivity(DTH) response in vivo were examined and the survival time of skin allograft was observed. RESULTS: Lymphocytes from Wistar rats that were pre fed with allogenetic SD rats splenocytes exhibited a significant reduction of mixed lymphocyte response in vitro and delayed type hypersensitivity response in vivo , compared with the unfed controls. While the unfed controls rejected the skin allografts within (4.9±0.1) days, the prefeds skin allograft survival time was (15 8±1 3) days.( P <0.01, n = 10). CONCLUSION: Orally administered alloantigen can down regulate the immune response to histocompatibility antigens, thus prolonging the survival time of skin allograft.