4、5、7月龄雌性大鼠骨计量学参数的变化及意义

Bone histomorphometric assay in female rats aged 4,5,and 7 months

目的 :观察 4、5和 7各月龄 SD♀大鼠的骨量、骨形成和骨吸收的变化。方法 :2 1只 3 .5月龄♀大鼠按随机排列表随机分为 3组 :4月龄组 6只、5月龄组 8只、7月龄组 7只。大鼠饲养至相应月龄时处死后做骨计量学分析。结果 :各组间骨小梁面积百分率 (% Tb.Ar)差异无显著性 ;5月龄组与 4月龄组比较 ,反映骨形成的参数矿化沉积率 (MAR)、骨形成率(BFR/ BS、BFR/ BV、BFR/ TV)都降低 ,7月龄组与 4月龄组比较 ,所有反映骨形成和骨吸收的参数都降低。结论 :在 4～ 7月龄期间 ,SD♀大鼠的骨形成和骨吸收活性下降 ,但仍保持平衡状态 。

Objective:To provide bone histomorphometric parameters in female rats.Methods:Twenty one female SD rats at the age of 3.5 months were randomly divided into 3 groups:4 month group( n =6),5 month group( n =8),7 month group( n =7).The rats in 3 groups were not sacrificed until they were fed at their respective months,and their proximal tibiae were assayed by histomorphometry.Results:There was no statistical significance in area of bone trabecula between 3 groups.Compared with 4 month group,the parameters of bone formation such as MAR,BFR/BS,BFR/BV and BFR/TV in 5 moth group decreased obviously,and all parameters that reflected bone formation and resorption in 7 month group lowered substantially.Conclusion:Though the bone formation and resorption lessened in female SD rats aged from 4 to 7 months,bone volume remains unchangeable.