大鼠出生前后胆碱补充对癫痫发作后认知功能的影响

Effects of pre- and postnatal choline supplementation on cognition impairments induced by seizures

目的 探讨出生前后胆碱补充或缺乏对癫痫发作后认知功能的影响。方法 3组大鼠自受孕后第 11天至子鼠出生后第 7天分别给予胆碱丰富、胆碱缺乏和正常胆碱含量的饮食。子鼠出生后第 42天 ,海人酸诱导癫痫发作。癫痫发作 10 d后 ,用 Morris水迷宫对大鼠空间学习记忆能力进行测试并测量海马中胆碱乙酰转移酶和乙酰胆碱酯酶的活性。结果 出生前后用胆碱丰富食物饲养的大鼠较胆碱缺乏饮食和胆碱正常饮食饲养的大鼠在水迷宫测试中有较好表现。经胆碱缺乏饮食饲养的大鼠海马中胆碱乙酰转移酶的活性较对照组低 18.8%,较胆碱补充组低 2 1.3%。结论 出生前后饮食中补充胆碱可以减轻癫痫发作引起的认知功能损害 ,这一作用可能与海马内胆碱乙酰转移酶的水平有关。

Objective This study is to evaluate the effects of pre- and postnatal choline supplementation on deficiency in cognitive dysfunction associated with seizures.Methods Choline supplemented, choline deficient and normal choline diet started from the 11th day of gestation to postnatal 7d. At postnatal 42d, rats were given a convulsant dosage of kainic acid (KA) to induce seizures. Ten days following KA induced seizures, rats underwent testing of visual-spatial learning and memory using Morris water maze. Choline acetyltranferase and acetylcholinsterase were measured after the behavioral testing.Results Rats received supplemental choline performed better in the water maze testing than the deficient and control groups. Moreover, the activity of hippocampal choline acetyltransferase was 18 8% lower in the choline deficient group as compared with control group and 21 3% lower as compared with choline supplemented group.Conclusion This study demonstrates that pre- and postnatal choline supplementation can protect rats from learning and memory deficits induced by seizures.