摘要
本实验用酶组织化学方法,观察服用雷公藤82糖浆和正常对照组小鼠睾丸组织中几种酶的变化。结果显示:给药6个月,曲精小管上皮的各级生精细胞中琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、三磷酸腺苷酶(ATPase)活性减弱;支持细胞中酸性磷酸酶(ACP)活性增强;碱性磷酸酶(AKP)活性,在给药组与对照组间无明显差别;睾丸间质细胞中SDH、ACP、AKP、ATPase等酶活性变化均不明显,但LDH活性增强。提示:雷公藤可抑制生精细胞中主要酶系的活性,进而导致精子发生和成熟的障碍。本文还就支持细胞中ACP活性增强做了讨论。
Testicular tissues of mice treated with Tripterygiura Wilfordii Hook F (TWH) were observed histochemically. The results showed that activity of several enzymes (SDH, LDH, ATPase) decreased and the distributions of these enzymes were in the spermatogenic cells, while the activity of ACP of Sertoli cells and LDH of Leydig cells increased, and there was no change in SDH, ATPase, ACP and AKP of Leydig cells and AKP of Sertoli cells. It is concluded that the effect of TWH is principally on the spermatogenic cells and it influences the development and growth of the spermatogenic cells.
基金
河北省计划生育委员会资助课题
关键词
睾丸
雷公藤
酶细胞化学
testis
Tripterygium Wilfordii Hook F
zymohistochemistry
mice
